Results 11 to 20 of about 30,137 (254)
GPCR structural characterization by NMR spectroscopy in solution
Acta Biochimica et Biophysica Sinica, 2022 In the human proteome, 826 G-protein-coupled receptors (GPCRs) interact with extracellular stimuli to initiate cascades of intracellular signaling. Determining conformational dynamics and intermolecular interactions are key to understand GPCR function as
Yang Lingyun +2 more
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Journal of Magnetic Resonance Open, 2022 In this work a rapid RNA assignment approach by combining chemical and enzymatic 13C and 15N stable isotope labeling is introduced. We exemplify the assignment strategy for imino N1H1 purine and N3H3 pyrimidine and aromatic C6H6 pyrimidine, C8H8 purine ...
David Klingler +3 more
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Scientific Data, 2023 Metabolic stable isotope labeling with heavy water followed by liquid chromatography coupled with mass spectrometry (LC-MS) is a powerful tool for in vivo protein turnover studies.
Henock M. Deberneh +8 more
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mfapy: An open-source Python package for 13C-based metabolic flux analysis
Metabolic Engineering Communications, 2021 13C-based metabolic flux analysis (13C-MFA) is an essential tool for estimating intracellular metabolic flux levels in metabolic engineering and biology.
Fumio Matsuda +6 more
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Stable-isotope labeling with amino acids in nematodes [PDF]
Nature Methods, 2011 We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline
Larance, Mark +8 more
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Metabolic Flux Analysis—Linking Isotope Labeling and Metabolic Fluxes
Metabolites, 2020 Metabolic flux analysis (MFA) is an increasingly important tool to study metabolism quantitatively. Unlike the concentrations of metabolites, the fluxes, which are the rates at which intracellular metabolites interconvert, are not directly measurable ...
Yujue Wang +4 more
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Stable isotope labelling of Ceratitis capitata [PDF]
Plant Protection Science, 2019 The use of stable isotopes to label an insect species, the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephidae) (medfly) was investigated. Labelling allows mating and life history characteristics to be investigated experimentally. 13C6-glucose was incorporated into the diet of medflies at various stages of development by adding it to larval ...
Hasan Al-Khshemawee +2 more
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Bio-Protocol, 2018 Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., 13C-glucose) in live animal is only beginning to be ...
Yuping Cai, Nan Liu, Zheng-Jiang Zhu
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Ultra-sensitive isotope probing to quantify activity and substrate assimilation in microbiomes
Microbiome, 2023 Background Stable isotope probing (SIP) approaches are a critical tool in microbiome research to determine associations between species and substrates, as well as the activity of species. The application of these approaches ranges from studying microbial
Manuel Kleiner +10 more
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Analytical Considerations of Stable Isotope Labelling in Lipidomics [PDF]
Biomolecules, 2018 Over the last two decades, lipids have come to be understood as far more than merely components of cellular membranes and forms of energy storage, and are now also being implicated to play important roles in a variety of diseases, with lipid biomarker research one of the most widespread applications of lipidomic techniques both in research and in ...
Alexander Triebl, Markus R. Wenk
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