Results 31 to 40 of about 30,977 (246)

Application of traditional and reverse labeling approaches to assess dietary bioavailability of metals in biofilms

Environment International
The accuracy of stable isotope tracing techniques, traditional and reverse labeling, in evaluating metal bioavailability remains uncertain. This study employed pulse-chase feeding combined with multi-heavy-metal stable isotope labeling to compare the ...
Yunuo Duan, Shiqi Zhu, Yuxuan Song, Dan Zhu, Mei Zhong, Liqiang Chen, Xia Luo   +6 more
doaj   +1 more source

Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments.

PLoS Computational Biology, 2015
Stable isotope labeling is the state of the art technique for in vivo quantification of lymphocyte kinetics in humans. It has been central to a number of seminal studies, particularly in the context of HIV-1 and leukemia.
Raya Ahmed, Liset Westera, Julia Drylewicz, Marjet Elemans, Yan Zhang, Elizabeth Kelly, Rajko Reljic, Kiki Tesselaar, Rob J de Boer, Derek C Macallan, José A M Borghans, Becca Asquith   +11 more
doaj   +1 more source

Stable isotope labeling strategy based on coding theory [PDF]

Journal of Biomolecular NMR, 2015
We describe a strategy for stable isotope-aided protein nuclear magnetic resonance (NMR) analysis, called stable isotope encoding. The basic idea of this strategy is that amino-acid selective labeling can be considered as "encoding and decoding" processes, in which the information of amino acid type is encoded by the stable isotope labeling ratio of ...
Kasai, Takuma, Koshiba, Seizo, Yokoyama, Jun, Kigawa, Takanori   +3 more
openaire   +2 more sources

A 13C isotope labeling method for the measurement of lignin metabolic flux in Arabidopsis stems

Plant Methods, 2018
Background Metabolic fluxes represent the functional phenotypes of biochemical pathways and are essential to reveal the distribution of precursors among metabolic networks. Although analysis of metabolic fluxes, facilitated by stable isotope labeling and
Peng Wang, Longyun Guo, Rohit Jaini, Antje Klempien, Rachel M. McCoy, John A. Morgan, Natalia Dudareva, Clint Chapple   +7 more
doaj   +1 more source

The Rules of Human T Cell Fate in vivo

Frontiers in Immunology, 2020
The processes governing lymphocyte fate (division, differentiation, and death), are typically assumed to be independent of cell age. This assumption has been challenged by a series of elegant studies which clearly show that, for murine cells in vitro ...
Pedro Costa del Amo, Bisrat Debebe, Milad Razavi-Mohseni, Shinji Nakaoka, Shinji Nakaoka, Andrew Worth, Diana Wallace, Peter Beverley, Derek Macallan, Becca Asquith   +9 more
doaj   +1 more source

Trajectories of plasma and CSF MTBR-tau243 and phosphorylated-tau species across the Alzheimer’s disease continuum

Nature Communications
To efficiently implement plasma and cerebrospinal fluid (CSF) biomarkers for staging and prognosis of Alzheimer disease (AD), we must understand their dynamics across disease progression.
Lyduine E. Collij, Gemma Salvadó, Kanta Horie, Nicolas R. Barthélemy, Tobey J. Betthauser, Olof Strandberg, Ruben Smith, Sebastian Palmqvist, Suzanne E. Schindler, Rik Ossenkoppele, Shorena Janelidze, Niklas Mattsson-Carlgren, Randall J. Bateman, Oskar Hansson   +13 more
doaj   +1 more source

An optimized method for measuring fatty acids and cholesterol in stable isotope-labeled cells

Journal of Lipid Research, 2017
Stable isotope labeling has become an important methodology for determining lipid metabolic parameters of normal and neoplastic cells. Conventional methods for fatty acid and cholesterol analysis have one or more issues that limit their utility for in ...
Joseph P. Argus, Amy K. Yu, Eric S. Wang, Kevin J. Williams, Steven J. Bensinger   +4 more
doaj   +1 more source

Springtime Drought Shifts Carbon Partitioning of Recent Photosynthates in 10-Year Old Picea mariana Trees, Causing Restricted Canopy Development

Frontiers in Forests and Global Change, 2021
Springtime bud-break and shoot development induces substantial carbon (C) costs in trees. Drought stress during shoot development can impede C uptake and translocation. This is therefore a channel through which water shortage can lead to restricted shoot
Anna M. Jensen, Diana Eckert, Kelsey R. Carter, Maria Persson, Jeffrey M. Warren   +4 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

18O Stable Isotope Labeling in MS-based Proteomics [PDF]

Briefings in Functional Genomics and Proteomics, 2009
A variety of stable isotope labeling techniques have been developed and used in mass spectrometry (MS)-based proteomics, primarily for relative quantitation of changes in protein abundances between two compared samples, but also for qualitative characterization of differentially labeled proteomes.
Xiaoying, Ye, Brian, Luke, Thorkell, Andresson, Josip, Blonder   +3 more
openaire   +2 more sources