Results 41 to 50 of about 30,977 (246)

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Synthesis and Characterization of Stable Isotope Labeled 3,3',4,4'-Tetrachlorobiphenyl-D6

Journal of Isotopes
A new synthetic method of the stable isotope labeled 3,3',4,4'-tetrachlorobiphenyl-D6 was investigated. The synthesis of 3,3',4,4'-tetrachlorobiphenyl-D6 is based on 1,2-dichlorobenzene-D4 as the labeling precursor through three steps of catalyzed ...
Rong WANG, Hui GUO, Wulian CHEN
doaj   +1 more source

Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism

Molecular Metabolism, 2019
Objective: The dynamic regulation of metabolic pathways can be monitored by stable isotope tracing. Yet, many metabolites are part of distinct processes within different subcellular compartments.
Sophie Trefely, Joyce Liu, Katharina Huber, Mary T. Doan, Helen Jiang, Jay Singh, Eliana von Krusenstiern, Anna Bostwick, Peining Xu, Juliane G. Bogner-Strauss, Kathryn E. Wellen, Nathaniel W. Snyder   +11 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

In Vivo Estimates of Liver Metabolic Flux Assessed by 13C-Propionate and 13C-Lactate Are Impacted by Tracer Recycling and Equilibrium Assumptions

Cell Reports, 2020
Summary: Isotope-based assessment of metabolic flux is achieved through a judicious balance of measurements and assumptions. Recent publications debate the validity of key assumptions used to model stable isotope labeling of liver metabolism in vivo ...
Clinton M. Hasenour, Mohsin Rahim, Jamey D. Young   +2 more
doaj   +1 more source

ZoomQuant: An application for the quantitation of stable isotope labeled peptides [PDF]

Journal of the American Society for Mass Spectrometry, 2005
The main goal of comparative proteomics is the quantitation of the differences in abundance of many proteins between two different biological samples in a single experiment. By differentially labeling the peptides from the two samples and combining them in a single analysis, relative ratios of protein abundance can be accurately determined.
Halligan, Brian D., Slyper, Ronit Y., Twigger, Simon N., Hicks, Wayne, Olivier, Michael, Greene, Andrew S.   +5 more
openaire   +2 more sources

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

iMS2Flux – a high–throughput processing tool for stable isotope labeled mass spectrometric data used for metabolic flux analysis

BMC Bioinformatics, 2012
Background Metabolic flux analysis has become an established method in systems biology and functional genomics. The most common approach for determining intracellular metabolic fluxes is to utilize mass spectrometry in combination with stable isotope ...
Poskar C Hart, Huege Jan, Krach Christian, Franke Mathias, Shachar-Hill Yair, Junker Björn H   +5 more
doaj   +1 more source

CSF Monoamine Metabolites and Cognitive Trajectory in Early Parkinson's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Imaging and postmortem studies indicate that abnormalities in monoaminergic neurotransmission contribute to cognitive impairment in Parkinson's disease (PD). However, it remains uncertain if cerebrospinal fluid (CSF) monoamine metabolites can serve as biomarkers of cognitive decline in early PD.
Jing‐Yu Shao, Rong Li, Yu‐Xiang Jiao, Kai Ma, Hong‐Qi Yang, Zheng‐Yu Sun, Jie‐Wen Zhang, Shuai Chen   +7 more
wiley   +1 more source

Escher-Trace: a web application for pathway-based visualization of stable isotope tracing data

BMC Bioinformatics, 2020
Background Stable isotope tracing has become an invaluable tool for probing the metabolism of biological systems. However, data analysis and visualization from metabolic tracing studies often involve multiple software packages and lack pathway ...
Avi Kumar, Jack Mitchener, Zachary A. King, Christian M. Metallo   +3 more
doaj   +1 more source