Results 21 to 30 of about 30,977 (246)

Stable isotope labeling methods for DNA

open access: yesProgress in Nuclear Magnetic Resonance Spectroscopy, 2016
NMR is a powerful method for studying proteins and nucleic acids in solution. The study of nucleic acids by NMR is far more challenging than for proteins, which is mainly due to the limited number of building blocks and unfavorable spectral properties.
Nelissen, Frank H.T.   +3 more
openaire   +4 more sources

Poly(3-Hydroxybutyrate) Biosynthesis from [U-13C6]D-Glucose by Ralstonia eutropha NCIMB 11599 and Recombinant Escherichia coli

open access: yesMicrobiology Research, 2023
The use of stable isotope-labeled polymers in in situ biodegradation tests provides detailed information on the degradation process. As isotope-labeled raw chemicals are generally expensive, it is desirable to prepare polymer samples with high production
Ramamoorthi M. Sivashankari   +2 more
doaj   +1 more source

Stable isotope labelling of Ceratitis capitata [PDF]

open access: yesPlant Protection Science, 2019
The use of stable isotopes to label an insect species, the Mediterranean fruit fly Ceratitis capitata (Diptera: Tephidae) (medfly) was investigated. Labelling allows mating and life history characteristics to be investigated experimentally. 13C6-glucose was incorporated into the diet of medflies at various stages of development by adding it to larval ...
Hasan Al-Khshemawee   +2 more
openaire   +2 more sources

gQuant, an Automated Tool for Quantitative Glycomic Data Analysis

open access: yesFrontiers in Chemistry, 2021
MALDI-MS-based glycan isotope labeling methods have been effectively and widely used for quantitative glycomics. However, interpretation of the data produced by MALDI-MS is inaccurate and tedious because the bioinformatic tools are inadequate.
Jiangming Huang   +7 more
doaj   +1 more source

Production of stable isotope-labeled acyl-coenzyme A thioesters by yeast stable isotope labeling by essential nutrients in cell culture [PDF]

open access: yesAnalytical Biochemistry, 2015
Acyl-coenzyme A (CoA) thioesters are key metabolites in numerous anabolic and catabolic pathways, including fatty acid biosynthesis and β-oxidation, the Krebs cycle, and cholesterol and isoprenoid biosynthesis. Stable isotope dilution-based methodology is the "gold standard" for quantitative analyses by mass spectrometry. However, chemical synthesis of
Nathaniel W, Snyder   +9 more
openaire   +2 more sources

Triple SILAC identified progestin-independent and dependent PRA and PRB interacting partners in breast cancer

open access: yesScientific Data, 2021
Measurement(s) progesterone receptor • isoform Technology Type(s) affinity purification method • stable isotope labeling by amino acids in cell culture Factor Type(s) progestin presence • biological replicate Machine-accessible metadata file describing ...
Prangwan Pateetin   +4 more
doaj   +1 more source

Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap

open access: yesShipin gongye ke-ji, 2023
Objective: To prevent some illegal companies driven by interests from arbitrarily labeling "organic" to deceive consumers using ways such as counterfeiting, forgery, and shoddy food.
Xuemei NIE, Yun XIE, Bozhou XU, Xiuli XU
doaj   +1 more source

Understanding Muscle Protein Dynamics: Technical Considerations for Advancing Sarcopenia Research [PDF]

open access: yesAnnals of Geriatric Medicine and Research, 2020
Sarcopenia, which is the loss of muscle mass and strength that occurs with aging, involves imbalanced muscle protein turnover (i.e., protein breakdown exceeding synthesis), which in turn exacerbates other clinical conditions such as type 2 diabetes ...
Il-Young Kim   +3 more
doaj   +1 more source

Stable isotope dimethyl labelling for quantitative proteomics and beyond [PDF]

open access: yesPhilosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2016
Stable-isotope reductive dimethylation, a cost-effective, simple, robust, reliable and easy-to- multiplex labelling method, is widely applied to quantitative proteomics using liquid chromatography-mass spectrometry. This review focuses on biological applications of stable-isotope dimethyl labelling for a large-scale comparative analysis of protein ...
Jue-Liang, Hsu, Shu-Hui, Chen
openaire   +2 more sources

Simultaneous Raman and Infrared Spectroscopy of Stable Isotope Labelled Escherichia coli

open access: yesSensors, 2022
We report the use of a novel technology based on optical photothermal infrared (O-PTIR) spectroscopy for obtaining simultaneous infrared and Raman spectra from the same location of the sample allowing us to study bacterial metabolism by monitoring the ...
Cassio Lima   +2 more
doaj   +1 more source

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