Results 21 to 30 of about 30,137 (254)

Stable isotope labeling methods for DNA

open access: yesProgress in Nuclear Magnetic Resonance Spectroscopy, 2016
NMR is a powerful method for studying proteins and nucleic acids in solution. The study of nucleic acids by NMR is far more challenging than for proteins, which is mainly due to the limited number of building blocks and unfavorable spectral properties.
Nelissen, Frank H.T.   +3 more
openaire   +4 more sources

Poly(3-Hydroxybutyrate) Biosynthesis from [U-13C6]D-Glucose by Ralstonia eutropha NCIMB 11599 and Recombinant Escherichia coli

open access: yesMicrobiology Research, 2023
The use of stable isotope-labeled polymers in in situ biodegradation tests provides detailed information on the degradation process. As isotope-labeled raw chemicals are generally expensive, it is desirable to prepare polymer samples with high production
Ramamoorthi M. Sivashankari   +2 more
doaj   +1 more source

gQuant, an Automated Tool for Quantitative Glycomic Data Analysis

open access: yesFrontiers in Chemistry, 2021
MALDI-MS-based glycan isotope labeling methods have been effectively and widely used for quantitative glycomics. However, interpretation of the data produced by MALDI-MS is inaccurate and tedious because the bioinformatic tools are inadequate.
Jiangming Huang   +7 more
doaj   +1 more source

Stable isotope metabolic labeling of Neisseria meningitidis lipooligosaccharide [PDF]

open access: yesJournal of Endotoxin Research, 2006
The lipooligosaccharide (LOS) of a Neisseria meningitidis acetate auxotroph was metabolically labeled with either [2-13C]-sodium acetate or [1,2-13C 2]-sodium acetate. In this study, we demonstrated that this label was efficiently incorporated into both the lipid A acyl moieties and the two N-acetylglucosamines present in the oligosaccharide branch of
Deborah M B, Post   +3 more
openaire   +2 more sources

Triple SILAC identified progestin-independent and dependent PRA and PRB interacting partners in breast cancer

open access: yesScientific Data, 2021
Measurement(s) progesterone receptor • isoform Technology Type(s) affinity purification method • stable isotope labeling by amino acids in cell culture Factor Type(s) progestin presence • biological replicate Machine-accessible metadata file describing ...
Prangwan Pateetin   +4 more
doaj   +1 more source

Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap

open access: yesShipin gongye ke-ji, 2023
Objective: To prevent some illegal companies driven by interests from arbitrarily labeling "organic" to deceive consumers using ways such as counterfeiting, forgery, and shoddy food.
Xuemei NIE, Yun XIE, Bozhou XU, Xiuli XU
doaj   +1 more source

18O Stable Isotope Labeling in MS-based Proteomics [PDF]

open access: yesBriefings in Functional Genomics and Proteomics, 2009
A variety of stable isotope labeling techniques have been developed and used in mass spectrometry (MS)-based proteomics, primarily for relative quantitation of changes in protein abundances between two compared samples, but also for qualitative characterization of differentially labeled proteomes.
Xiaoying, Ye   +3 more
openaire   +2 more sources

Multiplexed Analysis of Cage and Cage Free Chicken Egg Fatty Acids Using Stable Isotope Labeling and Mass Spectrometry

open access: yesMolecules, 2013
Binary stable isotope labeling couple with LC-ESI-MS has been used as a powerful non-targeted approach for the relative quantification of lipids, amino acids, and many other important metabolite classes.
Richard G. Torde   +4 more
doaj   +1 more source

Understanding Muscle Protein Dynamics: Technical Considerations for Advancing Sarcopenia Research [PDF]

open access: yesAnnals of Geriatric Medicine and Research, 2020
Sarcopenia, which is the loss of muscle mass and strength that occurs with aging, involves imbalanced muscle protein turnover (i.e., protein breakdown exceeding synthesis), which in turn exacerbates other clinical conditions such as type 2 diabetes ...
Il-Young Kim   +3 more
doaj   +1 more source

Benchmarking stable isotope labeling based quantitative proteomics

open access: yesJournal of Proteomics, 2013
Several quantitative mass spectrometry based technologies have recently evolved to interrogate the complexity, interconnectivity and dynamic nature of proteomes. Currently, the most popular methods use either metabolic or chemical isotope labeling with MS based quantification or chemical labeling using isobaric tags with MS/MS based quantification ...
A.F. Maarten Altelaar   +7 more
openaire   +6 more sources

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