Results 31 to 40 of about 30,137 (254)

Stable isotope labeling strategy based on coding theory [PDF]

Journal of Biomolecular NMR, 2015
We describe a strategy for stable isotope-aided protein nuclear magnetic resonance (NMR) analysis, called stable isotope encoding. The basic idea of this strategy is that amino-acid selective labeling can be considered as "encoding and decoding" processes, in which the information of amino acid type is encoded by the stable isotope labeling ratio of ...
Kasai, Takuma, Koshiba, Seizo, Yokoyama, Jun, Kigawa, Takanori   +3 more
openaire   +2 more sources

Application of traditional and reverse labeling approaches to assess dietary bioavailability of metals in biofilms

Environment International
The accuracy of stable isotope tracing techniques, traditional and reverse labeling, in evaluating metal bioavailability remains uncertain. This study employed pulse-chase feeding combined with multi-heavy-metal stable isotope labeling to compare the ...
Yunuo Duan, Shiqi Zhu, Yuxuan Song, Dan Zhu, Mei Zhong, Liqiang Chen, Xia Luo   +6 more
doaj   +1 more source

Simultaneous Raman and Infrared Spectroscopy of Stable Isotope Labelled Escherichia coli

Sensors, 2022
We report the use of a novel technology based on optical photothermal infrared (O-PTIR) spectroscopy for obtaining simultaneous infrared and Raman spectra from the same location of the sample allowing us to study bacterial metabolism by monitoring the ...
Cassio Lima, Howbeer Muhamadali, Royston Goodacre   +2 more
doaj   +1 more source

Anaerobic Mineralization of Stable-Isotope-Labeled 2-Methylnaphthalene [PDF]

Applied and Environmental Microbiology, 2001
ABSTRACT An active sulfate-reducing consortium that degrades 2-methylnaphthalene (2-MNAP) at rates of up to 25 μM day −1 was established. Degradation was inhibited in the presence of molybdate and ceased in the absence of sulfate. As much as 87% of 2-[ 14 C]MNAP was mineralized to
E R, Sullivan, X, Zhang, C, Phelps, L Y, Young   +3 more
openaire   +2 more sources

An optimized method for measuring fatty acids and cholesterol in stable isotope-labeled cells

Journal of Lipid Research, 2017
Stable isotope labeling has become an important methodology for determining lipid metabolic parameters of normal and neoplastic cells. Conventional methods for fatty acid and cholesterol analysis have one or more issues that limit their utility for in ...
Joseph P. Argus, Amy K. Yu, Eric S. Wang, Kevin J. Williams, Steven J. Bensinger   +4 more
doaj   +1 more source

Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments.

PLoS Computational Biology, 2015
Stable isotope labeling is the state of the art technique for in vivo quantification of lymphocyte kinetics in humans. It has been central to a number of seminal studies, particularly in the context of HIV-1 and leukemia.
Raya Ahmed, Liset Westera, Julia Drylewicz, Marjet Elemans, Yan Zhang, Elizabeth Kelly, Rajko Reljic, Kiki Tesselaar, Rob J de Boer, Derek C Macallan, José A M Borghans, Becca Asquith   +11 more
doaj   +1 more source

The Rules of Human T Cell Fate in vivo

Frontiers in Immunology, 2020
The processes governing lymphocyte fate (division, differentiation, and death), are typically assumed to be independent of cell age. This assumption has been challenged by a series of elegant studies which clearly show that, for murine cells in vitro ...
Pedro Costa del Amo, Bisrat Debebe, Milad Razavi-Mohseni, Shinji Nakaoka, Shinji Nakaoka, Andrew Worth, Diana Wallace, Peter Beverley, Derek Macallan, Becca Asquith   +9 more
doaj   +1 more source

A 13C isotope labeling method for the measurement of lignin metabolic flux in Arabidopsis stems

Plant Methods, 2018
Background Metabolic fluxes represent the functional phenotypes of biochemical pathways and are essential to reveal the distribution of precursors among metabolic networks. Although analysis of metabolic fluxes, facilitated by stable isotope labeling and
Peng Wang, Longyun Guo, Rohit Jaini, Antje Klempien, Rachel M. McCoy, John A. Morgan, Natalia Dudareva, Clint Chapple   +7 more
doaj   +1 more source

Springtime Drought Shifts Carbon Partitioning of Recent Photosynthates in 10-Year Old Picea mariana Trees, Causing Restricted Canopy Development

Frontiers in Forests and Global Change, 2021
Springtime bud-break and shoot development induces substantial carbon (C) costs in trees. Drought stress during shoot development can impede C uptake and translocation. This is therefore a channel through which water shortage can lead to restricted shoot
Anna M. Jensen, Diana Eckert, Kelsey R. Carter, Maria Persson, Jeffrey M. Warren   +4 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source