Results 41 to 50 of about 30,137 (254)

Synthesis and Characterization of Stable Isotope Labeled 3,3',4,4'-Tetrachlorobiphenyl-D6

Journal of Isotopes
A new synthetic method of the stable isotope labeled 3,3',4,4'-tetrachlorobiphenyl-D6 was investigated. The synthesis of 3,3',4,4'-tetrachlorobiphenyl-D6 is based on 1,2-dichlorobenzene-D4 as the labeling precursor through three steps of catalyzed ...
Rong WANG, Hui GUO, Wulian CHEN
doaj   +1 more source

Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism

Molecular Metabolism, 2019
Objective: The dynamic regulation of metabolic pathways can be monitored by stable isotope tracing. Yet, many metabolites are part of distinct processes within different subcellular compartments.
Sophie Trefely, Joyce Liu, Katharina Huber, Mary T. Doan, Helen Jiang, Jay Singh, Eliana von Krusenstiern, Anna Bostwick, Peining Xu, Juliane G. Bogner-Strauss, Kathryn E. Wellen, Nathaniel W. Snyder   +11 more
doaj   +1 more source

Escher-Trace: a web application for pathway-based visualization of stable isotope tracing data

BMC Bioinformatics, 2020
Background Stable isotope tracing has become an invaluable tool for probing the metabolism of biological systems. However, data analysis and visualization from metabolic tracing studies often involve multiple software packages and lack pathway ...
Avi Kumar, Jack Mitchener, Zachary A. King, Christian M. Metallo   +3 more
doaj   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

iMS2Flux – a high–throughput processing tool for stable isotope labeled mass spectrometric data used for metabolic flux analysis

BMC Bioinformatics, 2012
Background Metabolic flux analysis has become an established method in systems biology and functional genomics. The most common approach for determining intracellular metabolic fluxes is to utilize mass spectrometry in combination with stable isotope ...
Poskar C Hart, Huege Jan, Krach Christian, Franke Mathias, Shachar-Hill Yair, Junker Björn H   +5 more
doaj   +1 more source

In Vivo Estimates of Liver Metabolic Flux Assessed by 13C-Propionate and 13C-Lactate Are Impacted by Tracer Recycling and Equilibrium Assumptions

Cell Reports, 2020
Summary: Isotope-based assessment of metabolic flux is achieved through a judicious balance of measurements and assumptions. Recent publications debate the validity of key assumptions used to model stable isotope labeling of liver metabolism in vivo ...
Clinton M. Hasenour, Mohsin Rahim, Jamey D. Young   +2 more
doaj   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Discovery and Targeted Proteomic Studies Reveal Striatal Markers Validated for Huntington's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Clinical trials for Huntington's disease (HD) enrolling persons before clinical motor diagnosis (CMD) lack validated biomarkers. This study aimed to conduct an unbiased discovery analysis and a targeted examination of proteomic biomarkers scrutinized by clinical validation. Methods Cerebrospinal fluid was obtained from PREDICT‐HD and
Daniel Chelsky, Cara Joyce, H. Jeremy Bockholt, Paul A. Rudnick, William H. Adams, Fiona McAllister, Justin W. Smock, Michael A. Newton, Jane S. Paulsen   +8 more
wiley   +1 more source

Stable isotope dimethyl labelling for quantitative proteomics and beyond [PDF]

Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2016
Stable-isotope reductive dimethylation, a cost-effective, simple, robust, reliable and easy-to- multiplex labelling method, is widely applied to quantitative proteomics using liquid chromatography-mass spectrometry. This review focuses on biological applications of stable-isotope dimethyl labelling for a large-scale comparative analysis of protein ...
Jue-Liang, Hsu, Shu-Hui, Chen
openaire   +2 more sources

CSF Monoamine Metabolites and Cognitive Trajectory in Early Parkinson's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Imaging and postmortem studies indicate that abnormalities in monoaminergic neurotransmission contribute to cognitive impairment in Parkinson's disease (PD). However, it remains uncertain if cerebrospinal fluid (CSF) monoamine metabolites can serve as biomarkers of cognitive decline in early PD.
Jing‐Yu Shao, Rong Li, Yu‐Xiang Jiao, Kai Ma, Hong‐Qi Yang, Zheng‐Yu Sun, Jie‐Wen Zhang, Shuai Chen   +7 more
wiley   +1 more source