Results 61 to 70 of about 478,710 (331)
Acta Zoológica LilloanaThe study of sperm morphology involves the use of various staining techniques that allow visualization of different structures of the spermatozoon and their variabili- ty.
Ana Sofía Dip +3 more
doaj +1 more source
Method for observing Beauveria bassiana colonization in plants
MethodsX, 2021 Microbes interact in a multitude of ways with host plants, can dwell as endophytes within plants causing no apparent disease, and often provide benefits to their host.
Qiu-yang Wei, Yue-ni Liu
doaj +1 more source
Olig2/Plp-positive progenitor cells give rise to Bergmann glia in the cerebellum. [PDF]
, 2013 NG2 (nerve/glial antigen2)-expressing cells represent the largest population of postnatal progenitors in the central nervous system and have been classified as oligodendroglial progenitor cells, but the fate and function of these cells remain ...
Chung, S-H +4 more
core +2 more sources
FEBS Letters, EarlyView.In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
Journal of Isotopes, 2015 In continuation of the investigation on the bithiophene structure as potential Aβ probes, two halogenated chalcone derivatives containing bithiophene moiety were designed and evaluated as imaging probes for Aβ plaques.
PENG Cheng +3 more
doaj +1 more source
Quantitative determination of fluorescence labeling implemented in cell cultures
BMC Biology, 2023 Background Labeling efficiency is a crucial parameter in fluorescence applications, especially when studying biomolecular interactions. Current approaches for estimating the yield of fluorescent labeling have critical drawbacks that usually lead them to ...
Chiara Schirripa Spagnolo +4 more
doaj +1 more source
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus [PDF]
, 2009 The cellular uptake and localization of a Ru−octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a ...
Barton, Jacqueline K., Puckett, Cindy A.
core +3 more sources
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Early neuronal accumulation of DNA double strand breaks in Alzheimer's disease. [PDF]
, 2019 The maintenance of genomic integrity is essential for normal cellular functions. However, it is difficult to maintain over a lifetime in postmitotic cells such as neurons, in which DNA damage increases with age and is exacerbated by multiple neurological
Adame, Anthony +8 more
core +2 more sources