Results 31 to 40 of about 4,557 (217)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry

Journal of Lipid Research, 1999
A mass spectrometric method for the quantification of free cholesterol in cells and subcellular membranes is presented. The method is based on a simple one-step chemical derivatization of cholesterol to cholesterol-3-sulfate by a sulfur trioxide-pyridine
Roger Sandhoff, Britta Brügger, Dieter Jeckel, Wolf D. Lehmann, Felix T. Wieland   +4 more
doaj   +1 more source

Oil body extraction from oleo-proteaginous seeds and conservation of valuable native compounds☆

Oilseeds and fats, crops and lipids, 2023
Oil bodies, also called oleosomes have been the object of an increased interest since the last decade. Different processes of extraction and purification involve an aqueous crushing with methods to soften the cell membranes.
Fabre Jean-François, Lacroux Eric, Cerny Muriel, Vaca-Medina Guadalupe, Cassen Audrey, Merah Othmane, Valentin Romain, Mouloungui Zéphirin   +7 more
doaj   +1 more source

Unleashing the Power of Machine Learning in Nanomedicine Formulation Development

Advanced Functional Materials, EarlyView.
A random forest machine learning model is able to make predictions on nanoparticle attributes of different nanomedicines (i.e. lipid nanoparticles, liposomes, or PLGA nanoparticles) based on microfluidic formulation parameters. Machine learning models are based on a database of nanoparticle formulations, and models are able to generate unique solutions
Thomas L. Moore, Cristiano Pesce, Antonietta Greco, Claudia Pisante, Greta Avancini, Valentina Di Francesco, Yosi Shamay, Paolo Decuzzi   +7 more
wiley   +1 more source

Nanotherapies for Atherosclerosis: Targeting, Catalysis, and Energy Transduction

Advanced Healthcare Materials, EarlyView.
Atherosclerosis management is hindered by poor drug targeting and plaque heterogeneity. Nanotechnology overcomes these barriers via three core strategies: (1) target‐engineered nanocarriers that achieve lesion‐specific precision via ligand modification, biomimetic camouflage, stimuli‐responsive release, and self‐propelling nanomotors; (2) catalytic ...
Yuqi Yang, Jingguo Qu, Tonghuan Xiao, Xinyang Han, Michael Tin‐Cheung Ying   +4 more
wiley   +1 more source

(3β,16α)-3,16-Dihydroxypregn-5-en-20-one from the Twigs of Euonymus alatus (Thunb.) Sieb. Exerts Anti-Inflammatory Effects in LPS-Stimulated RAW-264.7 Macrophages

Molecules, 2019
To discover new pharmacologically active natural products, here, we performed the phytochemical analysis of a Korean medicinal plant. Euonymus alatus (Thunb.) Sieb.
Seulah Lee, Dahae Lee, Su Cheol Baek, Mun Seok Jo, Ki Sung Kang, Ki Hyun Kim   +5 more
doaj   +1 more source

Rapid Remodeling of the Human Gut Microbiome in Response to Short‐Term Animal Product Restriction and Associations with Host Molecular Phenotypes

Advanced Science, EarlyView.
A real‐world model of structured animal product restriction practiced for religious reasons reveals the dynamic adaptability of the human gut microbiome to dietary change and uncovers reductions in diversity and rare taxa loss. Integrated microbiome, metabolomic, and proteomic analyses uncover coordinated taxonomic and molecular shifts and identify ...
Christina Emmanouil, Maria Anezaki, Alexandros Simistiras, Stavros Glentis, Nikolaos Scarmeas, Pantelis Hatzis, Konstantinos Rouskas, Antigone S. Dimas   +7 more
wiley   +1 more source

Sterol and Triterpene Profiles of the Callus Culture of Solanum mammosum

Makara Journal of Science, 2019
This study aimed to compare the sterol and triterpene profiles of two types of Solanum mammosum callus cultures, i.e., compact globular structure (CGS) and normal fine (F) calluses. The CGS callus resulted from the differentiation of the F callus culture
Silvy Juliana, Suciati Suciati, Gunawan Indrayanto   +2 more
doaj   +1 more source

Intracellular sterol dynamics [PDF]

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2009
We review the cellular mechanisms implicated in cholesterol trafficking and distribution. Recent studies have provided new information about the distribution of sterols within cells, including analysis of its transbilayer distribution. The cholesterol interaction with other lipids and its engagement in various trafficking processes will determine its ...
Bruno, Mesmin, Frederick R, Maxfield
openaire   +2 more sources

Protection and Delivery of Phytochemicals from Passive Encapsulation to Guaranteed Self‐Assembly Induced by Amyloid Template for Chronic Disease Prevention via Modulating Microbial‐Host Crosstalk

Advanced Science, EarlyView.
ABSTRACT The rising incidence of chronic diseases globally has drawn widespread attention to phytochemicals, which exert targeted preventive and alleviating effect by modulating gut microbiota; thus, a potential strategy for precision nutritional interventions is offered.
Shiqi Bai, Siying Cheng, Fengguang Ma, Xiaorong Zhang, Jianan Huang, Zhonghua Liu, Bing Hu   +6 more
wiley   +1 more source