Results 41 to 50 of about 7,802 (179)

Eukaryotic ribosome recognizes 3′ context and stimulates stop codon readthrough [PDF]

, 2021
ABSTRACTIt is known that the nucleotide context surrounding stop codons significantly affects the efficiency of translation termination. In eukaryotes, various 3′ contexts have been described that are unfavourable for translation termination; however, the exact molecular mechanism that mediates their effect remains unknown.
Elizaveta Sokolova, Tatiana Egorova, Alexey Shuvalov, Elena Alkalaeva   +3 more
openaire   +1 more source

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals

eLife, 2014
Translational readthrough gives rise to low abundance proteins with C-terminal extensions beyond the stop codon. To identify functional translational readthrough, we estimated the readthrough propensity (RTP) of all stop codon contexts of the human ...
Fabian Schueren, Thomas Lingner, Rosemol George, Julia Hofhuis, Corinna Dickel, Jutta Gärtner, Sven Thoms   +6 more
doaj   +1 more source

Pharmaceuticals Promoting Premature Termination Codon Readthrough: Progress in Development

Biomolecules, 2023
Around 11% of all known gene lesions causing human genetic diseases are nonsense mutations that introduce a premature stop codon (PTC) into the protein-coding gene sequence.
Shan Li, Juan Li, Wenjing Shi, Ziyan Nie, Shasha Zhang, Fengdie Ma, Jun Hu, Jianjun Chen, Peiqiang Li, Xiaodong Xie   +9 more
doaj   +1 more source

Drug-induced eRF1 degradation promotes readthrough and reveals a new branch of ribosome quality control

Cell Reports, 2023
Summary: Suppression of premature termination codons (PTCs) by translational readthrough is a promising strategy to treat a wide variety of severe genetic diseases caused by nonsense mutations. Here, we present two potent readthrough promoters—NVS1.1 and
Lukas-Adrian Gurzeler, Marion Link, Yvonne Ibig, Isabel Schmidt, Olaf Galuba, Julian Schoenbett, Christelle Gasser-Didierlaurant, Christian N. Parker, Xiaohong Mao, Francis Bitsch, Markus Schirle, Philipp Couttet, Frederic Sigoillot, Jana Ziegelmüller, Anne-Christine Uldry, Wojciech Teodorowicz, Niko Schmiedeberg, Oliver Mühlemann, Jürgen Reinhardt   +18 more
doaj   +1 more source

Global analysis of translation termination in E. coli. [PDF]

PLoS Genetics, 2017
Terminating protein translation accurately and efficiently is critical for both protein fidelity and ribosome recycling for continued translation. The three bacterial release factors (RFs) play key roles: RF1 and 2 recognize stop codons and terminate ...
Natalie E Baggett, Yan Zhang, Carol A Gross   +2 more
doaj   +1 more source

A compact and simple method of achieving differential transgene expression by exploiting translational readthrough

BioTechniques, 2022
The development of multicistronic vectors enabling differential transgene expression is a goal of gene therapy and poses a significant engineering challenge. Current approaches rely on the insertion of long regulatory sequences that occupy valuable space
James E Sillibourne, Giulia Agliardi, Matteo Righi, Katerina Smetanova, Grant Rowley, Simon Speller, Abigail Dolor, Katarina Lamb, Christopher Allen, Rajeev Karattil, Farhaan Parekh, Frederick Arce Vargas, Simon Thomas, Shaun Cordoba, Martin Pule   +14 more
doaj   +1 more source

AMD1 mRNA employs ribosome stalling as a mechanism for molecular memory formation. [PDF]

, 2018
In addition to acting as template for protein synthesis, messenger RNA (mRNA) often contains sensory sequence elements that regulate this process1,2.
Andreev, Dmitry E., Atkins, John F., Baranov, Pavel V., Gladyshev, Vadim N., Kiniry, Stephen J., Loughran, Gary, Mariotti, Marco, Michel, Audrey M., O'Connor, Patrick B. F., Papkovsky, Dmitri B., Saffert, Paul, Tzani, Ioanna, Yordanova, Martina M., Zhdanov, Alexander V.   +13 more
core   +1 more source

An Equilibrium-Dependent Retroviral mRNA Switch Regulates Translational Recoding [PDF]

, 2011
Most retroviruses require translational recoding of a viral messenger RNA stop codon to maintain a precise ratio of structural (Gag) and enzymatic (Pol) proteins during virus assembly.
D'Souza, Victoria M, Durney, Michael Anthony, Goff, Stephen, Houck-Loomis, Brian, Nagle, Julia Marie, Salguero, Carolina, Shankar, Neelaabh   +6 more
core   +1 more source

Evidence of abundant stop codon readthrough in Drosophila and other metazoa [PDF]

Genome Research, 2011
While translational stop codon readthrough is often used by viral genomes, it has been observed for only a handful of eukaryotic genes. We previously used comparative genomics evidence to recognize protein-coding regions in 12 species of Drosophila and showed that for 149 genes, the open reading frame following the stop codon has a protein-coding ...
Jungreis, Irwin, Lin, Michael F., Spokony, Rebecca, Chan, Clara Sophia, Negre, Nicolas, Victorsen, Alec, White, Kevin P., Kellis, Manolis   +7 more
openaire   +3 more sources

Stops making sense: translational trade-offs and stop codon reassignment

BMC Evolutionary Biology, 2011
Background Efficient gene expression involves a trade-off between (i) premature termination of protein synthesis; and (ii) readthrough, where the ribosome fails to dissociate at the terminal stop.
Elgar Greg S, Lichtenstein Conrad P, Cotton James A, Johnson Louise J, Nichols Richard A, Polly p David, Le Comber Steven C   +6 more
doaj   +1 more source