Readthrough events in plants reveal plasticity of stop codons
Cell ReportsSummary: Stop codon readthrough (SCR) has important biological implications but remains largely uncharacterized. Here, we identify 1,009 SCR events in plants using a proteogenomic strategy. Plant SCR candidates tend to have shorter transcript lengths and
Yuqian Zhang +19 more
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Metabolic stress promotes stop-codon readthrough and phenotypic heterogeneity. [PDF]
Proc Natl Acad Sci U S A, 2020 Significance Protein synthesis is a fundamental cellular process that occurs from bacteria to humans. Highly accurate protein synthesis has been shown to be critical for the fitness of the cell and is controlled by sophisticated molecular mechanisms.
Zhang H +8 more
europepmc +4 more sources
Recognition of 3' nucleotide context and stop codon readthrough are determined during mRNA translation elongation. [PDF]
J Biol Chem, 2022 Biziaev N +6 more
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Aqp4 stop codon readthrough facilitates amyloid-β clearance from the brain. [PDF]
Brain, 2022 Abstract Alzheimer’s disease is initiated by the toxic aggregation of amyloid-β. Immunotherapeutics aimed at reducing amyloid beta are in clinical trials but with very limited success to date. Identification of orthogonal approaches for clearing amyloid beta may complement these approaches for treating Alzheimer’s disease.
Sapkota D +9 more
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Therapeutics based on stop codon readthrough. [PDF]
Annu Rev Genomics Hum Genet, 2014 Nonsense suppression therapy encompasses approaches aimed at suppressing translation termination at in-frame premature termination codons (PTCs, also known as nonsense mutations) to restore deficient protein function. In this review, we examine the current status of PTC suppression as a therapy for genetic diseases caused by nonsense mutations.
Keeling KM, Xue X, Gunn G, Bedwell DM.
europepmc +4 more sources
Deciphering the molecular mechanism of stop codon readthrough [PDF]
Biological Reviews, 2020 ABSTRACTRecognition of the stop codon by the translation machinery is essential to terminating translation at the right position and to synthesizing a protein of the correct size. Under certain conditions, the stop codon can be recognized as a coding codon promoting translation, which then terminates at a later stop codon. This event, called stop codon
Martine Palma, Fabrice Lejeune
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Increased expression of tryptophan and tyrosine tRNAs elevates stop codon readthrough of reporter systems in human cell lines. [PDF]
Nucleic Acids Res, 2021 Beznosková P +3 more
europepmc +2 more sources
Genome-scale quantification and prediction of pathogenic stop codon readthrough by small molecules. [PDF]
Nat GenetToledano I, Supek F, Lehner B.
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Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals. [PDF]
PLoS Genetics, 2014 Translation of mRNA into a polypeptide chain is a highly accurate process. Many prokaryotic and eukaryotic viruses, however, use leaky termination of translation to optimize their coding capacity.
Alina C Stiebler +6 more
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Statistical analysis of readthrough levels for nonsense mutations in mammalian cells reveals a major determinant of response to gentamicin. [PDF]
PLoS Genetics, 2012 The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for ...
Célia Floquet +3 more
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