Stop Codon Context-Specific Induction of Translational Readthrough [PDF]
Biomolecules, 2021 Premature termination codon (PTC) mutations account for approximately 10% of pathogenic variants in monogenic diseases. Stimulation of translational readthrough, also known as stop codon suppression, using translational readthrough-inducing drugs (TRIDs)
Mirco Schilff +3 more
doaj +7 more sources
Extended stop codon context predicts nonsense codon readthrough efficiency in human cells
Nature CommunicationsProtein synthesis terminates when a stop codon enters the ribosome’s A-site. Although termination is efficient, stop codon readthrough can occur when a near-cognate tRNA outcompetes release factors during decoding.
Kotchaphorn Mangkalaphiban +6 more
doaj +4 more sources
Evolutionary Dynamics of Abundant Stop Codon Readthrough. [PDF]
Mol Biol Evol, 2016 Translational stop codon readthrough emerged as a major regulatory mechanism affecting hundreds of genes in animal genomes, based on recent comparative genomics and ribosomal profiling evidence, but its evolutionary properties remain unknown. Here, we leverage comparative genomic evidence across 21 Anopheles mosquitoes to systematically annotate ...
Jungreis I +5 more
europepmc +8 more sources
eLife, 2020 Stop codon readthrough (SCR) occurs when the ribosome miscodes at a stop codon. Such readthrough events can be therapeutically desirable when a premature termination codon (PTC) is found in a critical gene.
Jamie R Wangen, Rachel Green
doaj +3 more sources
Versatile dual reporter gene systems for investigating stop codon readthrough in plants. [PDF]
PLoS ONE, 2009 Translation is most often terminated when a ribosome encounters the first in-frame stop codon (UAA, UAG or UGA) in an mRNA. However, many viruses (and some cellular mRNAs) contain "stop" codons that cause a proportion of ribosomes to terminate and others
Nga T Lao +3 more
doaj +4 more sources
Defining the high-translational readthrough stop codon context.
PLoS GeneticsTranslational termination is not entirely efficient and competes with elongation, which might result in translational readthrough (TR). TR occurs when a near-cognate tRNA binds to a stop codon, (mis)interpreting it as a sense codon and producing a C ...
Daniela Smoljanow +3 more
doaj +2 more sources
The minor gentamicin complex component, X2, is a potent premature stop codon readthrough molecule with therapeutic potential. [PDF]
PLoS ONE, 2018 Nonsense mutations, resulting in a premature stop codon in the open reading frame of mRNAs are responsible for thousands of inherited diseases. Readthrough of premature stop codons by small molecule drugs has emerged as a promising therapeutic approach ...
Westley J Friesen +20 more
doaj +2 more sources
BMC Medicine, 2007 Background Cystic fibrosis (CF) is caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) protein, which acts as a chloride channel activated by cyclic AMP (cAMP).
Reinert Philippe +12 more
doaj +3 more sources
A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing. [PDF]
PLoS PathogensIlarviruses are a relatively understudied but important group of plant RNA viruses that includes a number of crop pathogens. Their genomes comprise three RNA segments encoding two replicase subunits, movement protein, coat protein (CP), and (in some ...
Nina Lukhovitskaya +5 more
doaj +3 more sources
Ribosome profiling reveals pervasive and regulated stop codon readthrough in Drosophila melanogaster
eLife, 2013 Ribosomes can read through stop codons in a regulated manner, elongating rather than terminating the nascent peptide. Stop codon readthrough is essential to diverse viruses, and phylogenetically predicted to occur in a few hundred genes in Drosophila ...
Joshua G Dunn +4 more
doaj +3 more sources