Heterologous stop codon readthrough of metazoan readthrough candidates in yeast. [PDF]
PLoS ONE, 2013 Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the
Clara S Chan +2 more
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Transcriptome-wide investigation of stop codon readthrough in Saccharomyces cerevisiae. [PDF]
PLoS Genetics, 2021 Translation of mRNA into a polypeptide is terminated when the release factor eRF1 recognizes a UAA, UAG, or UGA stop codon in the ribosomal A site and stimulates nascent peptide release.
Kotchaphorn Mangkalaphiban +5 more
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The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code [PDF]
Open Biology, 2016 Translational readthrough gives rise to C-terminally extended proteins, thereby providing the cell with new protein isoforms. These may have different properties from the parental proteins if the extensions contain functional domains.
Julia Hofhuis +6 more
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Stop codon readthrough contexts influence reporter expression differentially depending on the presence of an IRES [version 3; peer review: 1 approved, 2 approved with reservations, 1 not approved] [PDF]
Wellcome Open Research, 2022 Background: Previously we reported the discovery of stop codon readthrough in AMD1 mRNA followed by ribosome stalling at the end of a conserved Open Reading Frame (ORF) that we termed AMD1.
Pavel V. Baranov +3 more
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Environment modulates protein heterogeneity through transcriptional and translational stop codon readthrough [PDF]
Nature CommunicationsStop codon readthrough events give rise to longer proteins, which may alter the protein’s function, thereby generating short-lasting phenotypic variability from a single gene.
Maria Luisa Romero Romero +6 more
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Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 system [PDF]
EMBO ReportsStop codon readthrough (SCR) is the process where translation continues beyond a stop codon on an mRNA. Here, we describe a strategy to enhance or induce SCR in a transcript-selective manner using a CRISPR-dCas13 system.
Lekha E Manjunath +6 more
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NanoLuc reporters identify COL4A5 nonsense mutations susceptible to drug-induced stop codon readthrough [PDF]
iScience, 2022 Summary: Alport syndrome, a disease of kidney, ear, and eye, is caused by pathogenic variants in the COL4A3, COL4A4, or COL4A5 genes encoding collagen α3α4α5(IV) of basement membranes.
Kohei Omachi +3 more
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Translation initiation factor eIF3 promotes programmed stop codon readthrough. [PDF]
Nucleic Acids Res, 2015 Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins.
Beznosková P +4 more
europepmc +3 more sources
Evidence of efficient stop codon readthrough in four mammalian genes. [PDF]
Nucleic Acids Res, 2014 Stop codon readthrough is used extensively by viruses to expand their gene expression. Until recent discoveries in Drosophila, only a very limited number of readthrough cases in chromosomal genes had been reported.
Loughran G +8 more
europepmc +5 more sources
3S-DB identifies an RNA repository facilitating stop codon readthrough for selenocysteine insertion and selenoproteome expansion [PDF]
Redox BiologySelenoproteins contain the selenocysteine (Sec, U), which is essential for redox regulation due to its reactive selenol group. The current set of 25 human selenoproteins was defined by the presence of SECIS elements in the 3’ UTR coupled with in-frame ...
Chenfang Si +7 more
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