Results 201 to 210 of about 87,040 (260)

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

The data archiving in the small herbaria digitisation workflow. [PDF]

Biodivers Data J
Novikov A.
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

A comprehensive method for quantifying human cytomegalovirus plaque assays. [PDF]

Front Cell Infect Microbiol
Kelnhofer-Millevolte LE, Nguyen DH, Wilson LS, Avgousti DC.   +3 more
europepmc   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

Protocol to isolate endosomal and small extracellular vesicles from cultured cells through ultracentrifugation. [PDF]

STAR Protoc
Domingues MN, Palhinhas L, Pereira P, Ferreira JV.   +3 more
europepmc   +1 more source

The Effects of Consumer’s Brand Awareness and Brand Familiarity on Store Loyalty in Apparel Stores: Causal Role of Brand Image, Store Image and Store Trust

Journal of Distribution and Management Research, 2017
openaire   +1 more source

Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing. [PDF]

STAR Protoc
Jiang X, Zhang W.
europepmc   +1 more source

Protocol for Nanodisc single-molecule pull-down assay to detect protein-lipid interactions. [PDF]

STAR Protoc
Reyes-Ordoñez A, Shree S, Sligar SG, Chen J.   +3 more
europepmc   +1 more source

Protocol for the quantification of digestive exophagy in cell culture. [PDF]

STAR Protoc
Funes L, Maxfield FR, Solé-Domènech S.
europepmc   +1 more source