Results 241 to 250 of about 236,402 (293)
Protein engineering of streptavidin for in vivo assembly of streptavidin beads
Journal of Biotechnology, 2008 Escherichia coli was engineered to intracellularly manufacture streptavidin beads. Variants of streptavidin (monomeric, core and mature full length streptavidin) were C-terminally fused to PhaC, the polyester granule forming enzyme of Cupriavidus necator. All streptavidin fusion proteins mediated formation of the respective granules in E. coli and were
Bernd Rehm
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Enzyme and Microbial Technology, 2011
Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on
Jae-Chul Pyun +2 more
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Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed by SDS-PAGE of the outer membrane proteins, and the number of autodisplayed streptavidin molecules on
Jae-Chul Pyun +2 more
exaly +3 more sources
Switchable reinforced streptavidin
Nanoscale, 2020Introducing a disulfide bridge within monovalent streptavidin's N-terminal β-sheet structure significantly increases the mechanical stability of the biotin/streptavidin complex.
Leonard C. Schendel +2 more
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Smart and biofunctional streptavidin
New Biotechnology, 1999The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. While it is extremely useful as the native protein, there are many applications where its function can be improved re-engineering the subunits.
Patrick Stayton +2 more
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Bioconjugate Chemistry, 1998
In this investigation, a comparison of wild type recombinant streptavidin (r-SAv) with two genetically engineered mutant r-SAv proteins was undertaken. The investigation also included a comparison of the r-SAv with two streptavidin (SAv) proteins from commercial sources. In vitro characterization of the SAv proteins was conducted by HPLC, SDS-PAGE, IEF,
D S, Wilbur +7 more
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In this investigation, a comparison of wild type recombinant streptavidin (r-SAv) with two genetically engineered mutant r-SAv proteins was undertaken. The investigation also included a comparison of the r-SAv with two streptavidin (SAv) proteins from commercial sources. In vitro characterization of the SAv proteins was conducted by HPLC, SDS-PAGE, IEF,
D S, Wilbur +7 more
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Smart Polymer–Streptavidin Conjugates
ChemInform, 2004AbstractFor Abstract see ChemInform Abstract in Full Text.
Patrick S, Stayton +2 more
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Avidin and streptavidin in clinical diagnostics
New Biotechnology, 1999With the introduction of the SA/Bi technology into the clinical diagnostic field ultrasensitive tests with an acceptable turn-around time became feasible. Cytokine ELISAs with lower detection limit in the picogram range became finally available. Prior to the introduction of SA/Bi only elevated levels of target could be detected by short time tests but ...
exaly +3 more sources
Streptavidin–biotin binding energetics
New Biotechnology, 1999The high affinity energetics in the streptavidin-biotin system provide an excellent model system for studying how proteins balance enthalpic and entropic components to generate an impressive overall free energy for ligand binding. We review here concerted site-directed mutagenesis, biophysical, and computational studies of aromatic and hydrogen bonding
Patrick Stayton +2 more
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Genetically engineered avidins and streptavidins
Cellular and Molecular Life Sciences, 2006Chicken avidin and bacterial streptavidin, (strept)avidin, are proteins widely utilized in a number of applications in life science, ranging from purification and labeling techniques to diagnostics, and from targeted drug delivery to nanotechnology. (Strept)avidin-biotin technology relies on the extremely tight and specific affinity between (strept ...
O H, Laitinen +3 more
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