Results 251 to 260 of about 236,402 (293)
Some of the next articles are maybe not open access.
Production of streptavidin in a synthetic medium
Journal of Immunological Methods, 1988A simple, inexpensive procedure for producing streptavidin has been described. The biotin-binding protein was produced by growing Streptomyces avidinii in a synthetic liquid culture medium containing L-asparagine as the sole nitrogen source. With this procedure, extraneous proteinaceous substances inherently present in culture media prepared with yeast
J, Cazin, M, Suter, J E, Butler
openaire +2 more sources
Ink Formulation for Inkjet Printing of Streptavidin and Streptavidin Functionalized Nanoparticles
Journal of Dispersion Science and Technology, 2011Aqueous inks containing the protein streptavidin (SAv) or nanoparticles carrying surface-attached SAv were formulated for piezoelectric inkjet printing, using glycerol and 2-propanol to adjust their dynamic viscosity and their surface tension by variation of concentration and relative ratio of the two organic components.
Kirsten Borchers +4 more
openaire +1 more source
Applied Spectroscopy, 2013
To characterize streptavidin immobilization on magnetic submicron particles (MSPs), residual streptavidin after magnetic removal of immobilized streptavidin was quantified with N-biotinyl- N′-(1-naphthyl)-ethylenediamine (BNEDA) based on Forster resonance energy transfer.
Gaobo, Long +6 more
openaire +2 more sources
To characterize streptavidin immobilization on magnetic submicron particles (MSPs), residual streptavidin after magnetic removal of immobilized streptavidin was quantified with N-biotinyl- N′-(1-naphthyl)-ethylenediamine (BNEDA) based on Forster resonance energy transfer.
Gaobo, Long +6 more
openaire +2 more sources
[Identification of streptavidin].
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao, 1996In this report, streptavidin (SA) was further identified on the basis of our previous researches in SA isolation and purification. 23.2 mg of SA productivity could be obtained after purification by DEAE-52 column from 1 L of culture supernatant. The spectrum scanning of ultraviolet absorption was congruent with that of the Sigma product.
W, Jia, J, Huang, W, Meng
openaire +1 more source
IET Synthetic Biology, 2007
The goal of this project was to express streptavidin on the outer surface of Escherichia coli so that we could target any biotinylated nucleic acid or peptide to a cell surface. We used BioBrick assembly to fuse together a lipoprotein signal peptide, outer membrane protein A transmembrane domains and streptavidin protein domains. Assembly of constructs
openaire +1 more source
The goal of this project was to express streptavidin on the outer surface of Escherichia coli so that we could target any biotinylated nucleic acid or peptide to a cell surface. We used BioBrick assembly to fuse together a lipoprotein signal peptide, outer membrane protein A transmembrane domains and streptavidin protein domains. Assembly of constructs
openaire +1 more source
Colloidal Gold/Streptavidin Methods
1994Biotin-avidin detection systems are widely used in both immunocytochemistry and molecular biology. They take advantage of the high affinity of biotin, a low-molecular-weight vitamin, for avidin, an egg-white protein. Because of the problem of nonspecific binding of avidin, streptavidin has largely replaced avidin for immunocytochemical procedures ...
openaire +4 more sources
Streptavidin and its biotin complex at atomic resolution
Acta Crystallographica Section D: Biological Crystallography, 2011Patrick Stayton +2 more
exaly