Results 41 to 50 of about 893,788 (266)

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Neutron structural biology

Journal of Physics and Chemistry of Solids, 1999
Hydrogen atoms and water molecules around proteins and nucleic acids play a crucial role in many physiological functions. Neutron diffraction provides an experimental method of directly locating hydrogen atoms. (a) Since almost all the H atom positions can be identified experimentally, the geometrical details of certain types of H-bonds can be ...
openaire   +3 more sources

Quantification of Intrinsically Disordered Proteins: A Problem Not Fully Appreciated

Frontiers in Molecular Biosciences, 2018
Protein quantification is essential in a great variety of biochemical assays, yet the inherent systematic errors associated with the concentration determination of intrinsically disordered proteins (IDPs) using classical methods are hardly appreciated ...
Sara Contreras-Martos, Sara Contreras-Martos, Hung H. Nguyen, Hung H. Nguyen, Phuong N. Nguyen, Phuong N. Nguyen, Nevena Hristozova, Nevena Hristozova, Mauricio Macossay-Castillo, Mauricio Macossay-Castillo, Denes Kovacs, Denes Kovacs, Angela Bekesi, Angela Bekesi, Jesper S. Oemig, Jesper S. Oemig, Dominique Maes, Kris Pauwels, Kris Pauwels, Peter Tompa, Peter Tompa, Peter Tompa, Pierre Lebrun, Pierre Lebrun   +23 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Structural biology of an organ

Cell Reports, 2023
Su et al.1 use a build-and-retrieve approach to both identify and determine structures of ten macromolecular machines in the human liver. The authors' method will launch researchers forward in understanding the structural biology of the cell (or organ).
openaire   +3 more sources

Structural Biology for Virus Research [PDF]

Frontiers in Microbiology, 2012
Viruses are strictly dependent on target host cells for their amplification. However, each virus has a unique strategy at each replication step, beginning with entry into the cell, followed by transcription, translation, assembly of viral genome/proteins, and finishing with the cellular release of progeny virions.
Miyazaki, Yasuyuki, Fujita, Mikako, Nomaguchi, Masako, Adachi, Akio   +3 more
openaire   +3 more sources

Disulfide-constrained Fabs overcome target size limitation for high-resolution single particle cryoEM

Nature Communications
High-resolution protein structures are essential for understanding biological mechanisms and drug discovery. While cryoEM has revolutionized structure determination of large protein complexes, most disease-related proteins are small (
Jennifer E. Kung, Matthew C. Johnson, Dimitry Tegunov, Christine C. Jao, Ping Wu, Angela Oh, May Lin, Jose M. Daria, Christopher M. Koth, Christopher P. Arthur, Alexis Rohou, Jawahar Sudhamsu   +11 more
doaj   +1 more source

OffsampleAI: artificial intelligence approach to recognize off-sample mass spectrometry images

BMC Bioinformatics, 2020
Background Imaging mass spectrometry (imaging MS) is an enabling technology for spatial metabolomics of tissue sections with rapidly growing areas of applications in biology and medicine.
Katja Ovchinnikova, Vitaly Kovalev, Lachlan Stuart, Theodore Alexandrov   +3 more
doaj   +1 more source

Chromatin reconstruction during mouse terminal erythropoiesis

iScience, 2022
Summary: Mammalian terminal erythropoiesis involves chromatin and nuclear condensation followed by enucleation. Late-stage erythroblasts undergo caspase-mediated nuclear opening that is important for nuclear condensation through partial histone release ...
Honghao Bi, Ye Hou, Juan Wang, Zongjun Xia, Dongmei Wang, Yijie Liu, Haiyan Bao, Xu Han, Kehan Ren, Ermin Li, Feng Yue, Peng Ji   +11 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source