Results 61 to 70 of about 5,504,723 (310)

Quantification of Intrinsically Disordered Proteins: A Problem Not Fully Appreciated

Frontiers in Molecular Biosciences, 2018
Protein quantification is essential in a great variety of biochemical assays, yet the inherent systematic errors associated with the concentration determination of intrinsically disordered proteins (IDPs) using classical methods are hardly appreciated ...
Sara Contreras-Martos, Sara Contreras-Martos, Hung H. Nguyen, Hung H. Nguyen, Phuong N. Nguyen, Phuong N. Nguyen, Nevena Hristozova, Nevena Hristozova, Mauricio Macossay-Castillo, Mauricio Macossay-Castillo, Denes Kovacs, Denes Kovacs, Angela Bekesi, Angela Bekesi, Jesper S. Oemig, Jesper S. Oemig, Dominique Maes, Kris Pauwels, Kris Pauwels, Peter Tompa, Peter Tompa, Peter Tompa, Pierre Lebrun, Pierre Lebrun   +23 more
doaj   +1 more source

Structural insights into aquaporin selectivity and regulation

, 2015
Aquaporins have emerged as one of the structurally best-characterized membrane protein families, with fourteen different structures available from a diverse range of organisms.
Kreida, Stefan,, Lund University., Horsefield, Susanna,   +2 more
core   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Disulfide-constrained Fabs overcome target size limitation for high-resolution single particle cryoEM

Nature Communications
High-resolution protein structures are essential for understanding biological mechanisms and drug discovery. While cryoEM has revolutionized structure determination of large protein complexes, most disease-related proteins are small (
Jennifer E. Kung, Matthew C. Johnson, Dimitry Tegunov, Christine C. Jao, Ping Wu, Angela Oh, May Lin, Jose M. Daria, Christopher M. Koth, Christopher P. Arthur, Alexis Rohou, Jawahar Sudhamsu   +11 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

OffsampleAI: artificial intelligence approach to recognize off-sample mass spectrometry images

BMC Bioinformatics, 2020
Background Imaging mass spectrometry (imaging MS) is an enabling technology for spatial metabolomics of tissue sections with rapidly growing areas of applications in biology and medicine.
Katja Ovchinnikova, Vitaly Kovalev, Lachlan Stuart, Theodore Alexandrov   +3 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Structural and dynamical investigation of histone H2B in well-hydrated nucleosome core particles by solid-state NMR

, 2023
H2A-H2B dimer is a key component of nucleosomes and an important player in chromatin biology. Here, we characterized the structure and dynamics of H2B in precipitated nucleosome core particles (NCPs) with a physiologically relevant concentration using ...
Prasanna, Chinmayi, Nordenskiöld, Lars, Bhuvaneswari Kannaian, Kannaian, Bhuvaneswari, Chinmayi Prasanna, Xiangyan Shi, Aghil Soman, Soman, Aghil, Shi, Xiangyan, Lars Nordenskiöld   +9 more
core   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Structural Biology of Zinc

, 1991
The biological function of zinc is governed by the composition of its tetrahedral coordination polyhedron in the metalloprotein, and each ligand group that coordinates to the metal ion does so with a well-defined stereochemical preference. Consequently, protein-zinc recognition and discrimination requires proper chemical composition and proper ...
openaire   +2 more sources