Results 71 to 80 of about 893,788 (266)

Diffraction Techniques in Structural Biology [PDF]

Current Protocols in Nucleic Acid Chemistry, 2010
AbstractA detailed understanding of chemical and biological function and the mechanisms underlying the molecular activities ultimately requires atomic‐resolution structural data. Diffraction‐based techniques such as single‐crystal X‐ray crystallography, electron microscopy, and neutron diffraction are well established and they have paved the road to ...
openaire   +4 more sources

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Decoding phospho-regulation and flanking regions in autophagy-associated short linear motifs

Communications Biology
Short Linear Motifs (SLiMs) play a pivotal role in the interactions between intrinsically disordered proteins and their binding partners. SLiMs can undergo regulation through post-translational modifications, including phosphorylation.
Mattia Utichi, Oana N. Antonescu, Valentina Sora, Henri-Baptiste Marjault, Matteo Tiberti, Emiliano Maiani, Matteo Lambrughi, Elena Papaleo   +7 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

The structural biology of PRRSV

Virus Research, 2010
Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped, positive-sense single-stranded RNA virus belonging to the Arteriviridae family. Arteriviruses and coronaviruses are grouped together in the order Nidovirales, based on similarities in genome organization and expression strategy.
openaire   +2 more sources

Pmt4 recognizes two separate acceptor sites to O-mannosylate in the S/T-rich regions of substrate proteins

Nature Communications
Protein O-mannosyltransferases (PMTs) add mannose to serine/threonine (S/T)-rich proteins in the endoplasmic reticulum, facilitating proper folding and trafficking through the secretory pathway.
Minge Du, Zuanning Yuan, Amanda Kovach, Meinan Lyu, Huilin Li   +4 more
doaj   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

Speckle-modulating optical coherence tomography in living mice and humans

Nature Communications, 2017
Optical coherence tomography, a technique that can image inside tissue, is susceptible to speckle noise that limits its diagnostic potential. Here, Libaet al.
Orly Liba, Matthew D. Lew, Elliott D. SoRelle, Rebecca Dutta, Debasish Sen, Darius M. Moshfeghi, Steven Chu, Adam de la Zerda   +7 more
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source