Results 1 to 10 of about 10,680,823 (279)

The Substrate Specificity of Sirtuins

Annual Review of Biochemistry, 2016
Sirtuins are NAD+-dependent enzymes universally present in all organisms, where they play central roles in regulating numerous biological processes. Although early studies showed that sirtuins deacetylated lysines in a reaction that consumes NAD+, more recent studies have revealed that these enzymes can remove a variety of acyl-lysine modifications ...
Bheda, P., Jing, H., Wolberger, C., Lin, H.   +3 more
openaire   +4 more sources

The intrinsic substrate specificity of the human tyrosine kinome. [PDF]

Nature
Phosphorylation of proteins on tyrosine (Tyr) residues evolved in metazoan organisms as a mechanism of coordinating tissue growth1. Multicellular eukaryotes typically have more than 50 distinct protein Tyr kinases that catalyse the phosphorylation of ...
Yaron-Barir TM, Joughin BA, Huntsman EM, Kerelsky A, Cizin DM, Cohen BM, Regev A, Song J, Vasan N, Lin TY, Orozco JM, Schoenherr C, Sagum C, Bedford MT, Wynn RM, Tso SC, Chuang DT, Li L, Li SS, Creixell P, Krismer K, Takegami M, Lee H, Zhang B, Lu J, Cossentino I, Landry SD, Uduman M, Blenis J, Elemento O, Frame MC, Hornbeck PV, Cantley LC, Turk BE, Yaffe MB, Johnson JL.   +35 more
europepmc   +2 more sources

Structural basis for substrate specificity of mammalian neuraminidases.

PLoS ONE, 2014
The removal of sialic acid (Sia) residues from glycoconjugates in vertebrates is mediated by a family of neuraminidases (sialidases) consisting of Neu1, Neu2, Neu3 and Neu4 enzymes.
Victoria Smutova, Amgad Albohy, Xuefang Pan, Elena Korchagina, Taeko Miyagi, Nicolai Bovin, Christopher W Cairo, Alexey V Pshezhetsky   +7 more
doaj   +2 more sources

Probabilistic approach to predicting substrate specificity of methyltransferases.

PLoS Computational Biology, 2014
We present a general probabilistic framework for predicting the substrate specificity of enzymes. We designed this approach to be easily applicable to different organisms and enzymes.
Teresa Szczepińska, Jan Kutner, Michał Kopczyński, Krzysztof Pawłowski, Andrzej Dziembowski, Andrzej Kudlicki, Krzysztof Ginalski, Maga Rowicka   +7 more
doaj   +2 more sources

Phage-assisted continuous evolution of proteases with altered substrate specificity

Nature Communications, 2017
Proteases are promising therapeutics to treat diseases such as hemophilia which are due to endogenous protease deficiency. Here the authors use phage-assisted continuous evolution to evolve a variant TEV protease with altered target peptide sequence ...
Michael S. Packer, Holly A. Rees, David R. Liu   +2 more
doaj   +2 more sources

Structural basis of SIRT7 nucleosome engagement and substrate specificity [PDF]

Nature Communications
Chromatin-modifying enzymes target distinct residues within histones to finetune gene expression profiles. SIRT7 is an NAD+-dependent deacylase often deregulated in cancer, which deacetylates either H3 lysine 36 (H3K36) or H3K18 with high specificity ...
Carlos Moreno-Yruela, Babatunde E. Ekundayo, Polina N. Foteva, Dongchun Ni, Esther Calvino-Sanles, Henning Stahlberg, Beat Fierz   +6 more
doaj   +3 more sources

Substrate Specificity in Thiol Dioxygenases

Biochemistry, 2019
Thiol dioxygenases make up a class of ferrous iron-dependent enzymes that oxidize thiols to their corresponding sulfinates. X-ray diffraction structures of cysteine-bound cysteine dioxygenase show how cysteine is coordinated via its thiolate and amine to the iron and oriented correctly for O atom transfer.
Sekotilani Aloi, Casey G. Davies, P. Andrew Karplus, Sigurd M. Wilbanks, Guy N. L. Jameson   +4 more
openaire   +3 more sources

Discovery and substrate specificity engineering of nucleotide halogenases [PDF]

Nature Communications
C2′-halogenation has been recognized as an essential modification to enhance the drug-like properties of nucleotide analogs. The direct C2ʹ-halogenation of the nucleotide 2′-deoxyadenosine-5′-monophosphate (dAMP) has recently been achieved using the Fe ...
Jie Ni, Jingyuan Zhuang, Yiming Shi, Ying-Chih Chiang, Gui-Juan Cheng   +4 more
doaj   +2 more sources

Structural Analysis and Substrate Specificity of D-Carbamoylase from Pseudomonas [PDF]

BioTech
The synthesis of enantiomeric forms of D-amino acids can be achieved by a two-step “hydantoinase process” based on the sequential catalysis of substrates by specific enzymes, D-carbamoylase and D-hydantoinase. Here, we describe the structural features of
Marina Paronyan, Haykanush Koloyan, Hovsep Aganyants, Artur Hambardzumyan, Tigran Soghomonyan, Sona Avetisyan, Sergey Kocharov, Henry Panosyan, Vehary Sakanyan, Anichka Hovsepyan   +9 more
doaj   +2 more sources

Substrate Specificity of SARS-CoV-2 Nsp10-Nsp16 Methyltransferase

Viruses, 2021
The ongoing COVID-19 pandemic exemplifies the general need to better understand viral infections. The positive single-strand RNA genome of its causative agent, the SARS coronavirus 2 (SARS-CoV-2), encodes all viral enzymes.
Roberto Benoni, Petra Krafcikova, Marek R. Baranowski, Joanna Kowalska, Evzen Boura, Hana Cahová   +5 more
doaj   +1 more source