Results 21 to 30 of about 10,438,012 (377)

Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases

Nature Communications, 2021
Pinoresinol–lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis. Here, crystal structures of three PLRs in the apo, substrate-bound and product-bound states, and accompanying mutagenesis provide insight into PLRs catalytic ...
Ying Xiao, Kai Shao, Jingwen Zhou, Lian Wang, Xueqi Ma, Di Wu, Yingbo Yang, Junfeng Chen, Jingxian Feng, Shi Qiu, Zongyou Lv, Lei Zhang, Peng Zhang, Wansheng Chen   +13 more
doaj   +1 more source

The substrate specificity of phospholipase A

Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects, 1963
Investigations on variously modified analogues of phospholipids elucidated the following substrate characteristics for phospholipase A (Crotalus adamanteus). 1. 1. Within the class of α-phosphoglycerides l-isomers are readily hydrolysed, while d-α-phospholipids appeared not to be attacked. 2. 2.
Deenen, L.L.M. van, Haas, Gerard H. de
openaire   +3 more sources

Substrate Specificity of the SecB Chaperone [PDF]

Journal of Biological Chemistry, 1999
The bacterial chaperone SecB assists translocation of proteins across the inner membrane. The mechanism by which it differentiates between secretory and cytosolic proteins is poorly understood. To identify its binding motif, we screened 2688 peptides covering sequences of 23 proteins for SecB binding.
Knoblauch, Nicola T. M., Rüdiger, Stefan, Schönfeld, Hans-Joachim, Driessen, Arnold J. M., Schneider-Mergener, Jens, Bukau, Bernd   +5 more
openaire   +5 more sources

Substrate Specificity of Biofilms Proximate to Historic Shipwrecks

Microorganisms, 2023
The number of built structures on the seabed, such as shipwrecks, energy platforms, and pipelines, is increasing in coastal and offshore regions. These structures, typically composed of steel or wood, are substrates for microbial attachment and biofilm ...
Rachel L. Mugge, Rachel D. Moseley, Leila J. Hamdan   +2 more
doaj   +1 more source

Phosphotyrosine Substrate Sequence Motifs for Dual Specificity Phosphatases. [PDF]

PLoS ONE, 2015
Protein tyrosine phosphatases dephosphorylate tyrosine residues of proteins, whereas, dual specificity phosphatases (DUSPs) are a subgroup of protein tyrosine phosphatases that dephosphorylate not only Tyr(P) residue, but also the Ser(P) and Thr(P ...
Bryan M Zhao, Sarah L Keasey, Joseph E Tropea, George T Lountos, Beverly K Dyas, Scott Cherry, Sreejith Raran-Kurussi, David S Waugh, Robert G Ulrich   +8 more
doaj   +1 more source

Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?

Biomolecules, 2023
Amino acid antiporters mediate the 1:1 exchange of groups of amino acids. Whether substrate specificity can be different for the inward and outward facing conformation has not been investigated systematically, although examples of asymmetric transport ...
Gregory Gauthier-Coles, Stephen J. Fairweather, Angelika Bröer, Stefan Bröer   +3 more
doaj   +1 more source

Differential PROTAC substrate specificity dictated by orientation of recruited E3 ligase

Nature Communications, 2019
PROteolysis-TArgeting Chimeras (PROTACs) are hetero-bifunctional molecules that recruit an E3 ubiquitin ligase to a given substrate protein resulting in its targeted degradation.
Blake E. Smith, Stephen L. Wang, S. Jaime-Figueroa, A. Harbin, Jing Wang, B. Hamman, C. Crews   +6 more
semanticscholar   +1 more source

The Substrate Specificity of Fumarase

Journal of Biological Chemistry, 1968
Abstract The substrate specificity of fumarase has been studied by measuring the ability of the enzyme to hydrate or dehydrate derivatives of fumarate and l-malate. Fumarase was observed to hydrate fumarate and its derivatives in the order, fluorofumarate g fumarate g chlorofumarate g bromofumarate g acetylenedicarboxylate g iodofumarate g mesaconate ...
G M Hass, Robert L. Hill, John W. Teipel
openaire   +3 more sources

Substrate specificity of human ceramide kinase

Journal of Lipid Research, 2005
Previous studies in our laboratory have established ceramide kinase (CERK) as a critical mediator of eicosanoid synthesis. To date, CERK has not been well characterized in vitro.
Dayanjan S. Wijesinghe, Autumn Massiello, Preeti Subramanian, Zdzislaw Szulc, Alicja Bielawska, Charles E. Chalfant   +5 more
doaj   +1 more source

Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate

Biomolecules, 2020
The cytochrome P450 OleP catalyzes the epoxidation of aliphatic carbons on both the aglycone 8.8a-deoxyoleandolide (DEO) and the monoglycosylated L-olivosyl-8.8a-deoxyoleandolide (L-O-DEO) intermediates of oleandomycin biosynthesis.
Giacomo Parisi, Ida Freda, Cécile Exertier, Cristina Cecchetti, Elena Gugole, Gabriele Cerutti, Lucia D’Auria, Alberto Macone, Beatrice Vallone, Carmelinda Savino, Linda Celeste Montemiglio   +10 more
doaj   +1 more source