Structure-function analysis of plant fructosyltransferases [PDF]
Fructans are an important class of plant carbohydrates that consist of linear or branched chains of fructosyl moieties. Their synthesis requires fructosyltransferases (FTs) that catalyze the transfer of fructosyl units from a donor substrate (sucrose ...
Altenbach, Denise
core +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A useful quantitative model for determination of enantiomeric composition of racemate praziquantel by ultraviolet spectroscopy combined with partial least squares and its application to praziquantel tablets [PDF]
A simple and novel method has been proposed to determine the enantiomeric composition of racemate praziquantel (PZQ) by using the analysis of ultraviolet (UV) spectroscopy combined with partial least squares (PLS). This method does not rely on the use of
Man Zhao +6 more
doaj +1 more source
Epilepsy‐Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties
ABSTRACT Objective Pathogenic variants in SCN1A, which encodes the voltage‐gated sodium channel NaV1.1, are associated with multiple epilepsy syndromes exhibiting a range of clinical severity. SCN1A variants are reported in different syndromes, including Dravet syndrome, which is associated with loss‐of‐function, whereas neonatal/infantile‐onset ...
Lanie N. Liebovitz +3 more
wiley +1 more source
In order to improve Timor deer (Cervus timorensis) frozen semen quality, the influence of sugar and glycerol concentration on semen characteristics of sperm was investigated.
W. M. M. Nalley +5 more
doaj
Studies on sucrose phosphate synthetase [PDF]
Plant biochemists are confronted with two path- ways leading to sucrose, both of which involve UDP- glucose as glucosyl donor. Sucrose synthetase [l] (UDP-glucose: D-fructose-2glucosyltransferase, EC 2.4.2.13) and sucrose phosphate synthetase [2] (UDP-glucose: D-fructose-6-phosphate-Zglucosyl- transferase, EC 2.4.2.14) with its associate sucrose ...
Salerno, Graciela L., Pontis, Horacio G.
openaire +2 more sources
The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett +19 more
wiley +1 more source
Dissociation of place preference and tolerance responses to sucrose using a dopamine antagonist in the planarian [PDF]
In rodents, sucrose has been found to elicit addictive-like behaviours like the development of tolerance and the association with cues present at the time of consumption.
Prados, J.
core +1 more source
Implantable Ionic Memristors Based on Natural Polymer Heterojunctions
We report an implantable natural polymer‐based ionic memristor composed of hyaluronic acid, chitosan, and PDMS. The device achieved 98.94% accuracy in MNIST classification while reducing training time by 36.8% compared with a conventional artificial neural network (ANN).
Dong‐yup Lee +6 more
wiley +1 more source

