Results 91 to 100 of about 6,413 (224)

L7Ae- und LSm-RNA-Interaktome von Sulfolobus acidocaldarius

, 2017
Die archaealen Proteine L7Ae und LSm (Sm-ähnliche Proteine) sind universelle RNA-Bindeproteine. L7Ae stabilisiert nicht-kodierende RNAs, einschließlich ribosomaler RNA, durch Erkennung eines RNA-Strukturmotivs, namens „kink-turn“ (k-turn). Proteine der Sm-Familie, wie die bakteriellen Hfq und die eukaryotischen Sm/LSm-Proteine, sind an multiplen RNA ...
Daume, Michael, Randau, Lennart (Dr.)
openaire   +3 more sources

Designing Enzymatic Reactivity with an Expanded Palette

ChemBioChem, Volume 26, Issue 11, June 3, 2025.
Innovation in biocatalysis is rapidly increasingly the diversity of catalytic reactivity that can be mediated by enzymes, addressing a key bottleneck for their widespread adoption in industrial chemical synthesis. A key approach to this is building enzymes with unnatural catalytic components that provide an expanded palette with new possibilities for ...
Reuben B. Leveson‐Gower
wiley   +1 more source

Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.

PLoS ONE, 2013
Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In
Birgit Märtens, Fabian Amman, Salim Manoharadas, Lukas Zeichen, Alvaro Orell, Sonja-Verena Albers, Ivo Hofacker, Udo Bläsi   +7 more
doaj   +1 more source

Involvement of a eukaryotic-like ubiquitin-related modifier in the proteasome pathway of the archaeon Sulfolobus acidocaldarius. [PDF]

, 2015
In eukaryotes, the covalent attachment of ubiquitin chains directs substrates to the proteasome for degradation. Recently, ubiquitin-like modifications have also been described in the archaeal domain of life.
Albers, Sonja-Verena, Anjum, Rana S, Blackwood, John K, Bray, Sian M, Coelho, Matthew A, Deery, Michael J, Foster, Benjamin M, Howard, Julie A, Kilkenny, Mairi L, Li, Shurong, Pellegrini, Luca, Robinson, Nicholas P   +11 more
core   +2 more sources

Energy flux controls tetraether lipid cyclization in Sulfolobus acidocaldarius [PDF]

Environmental Microbiology, 2019
Summary Microorganisms regulate the composition of their membranes in response to environmental cues. Many Archaea maintain the fluidity and permeability of their membranes by adjusting the number of cyclic moieties within the cores of their glycerol dibiphytanyl glycerol tetraether (GDGT) lipids.
Alice Zhou, Yuki Weber, Beverly K. Chiu, Felix J. Elling, Alec B. Cobban, Ann Pearson, William D. Leavitt   +6 more
openaire   +3 more sources

Engineering and Structural Elucidation of a Sac7d‐Derived IgG Fc‐Specific Affitin and Its Application for the Light‐Controlled Affinity Purification of Antibodies

ChemBioChem, Volume 26, Issue 11, June 3, 2025.
An affitin derived from the Sac7d scaffold enables the light‐controlled affinity purification of antibodies under physiological conditions, thus overcoming limitations of protein A chromatography. X‐ray crystallographic analysis and affinity measurements reveal complex formation with Fc at the CH2/CH3 junction, similar to protein A/G and FnRn. Combined
Felix Veitl, Andreas Eichinger, Peter Mayrhofer, Markus R. Anneser, Mauricio Testanera, Kilian Rauscher, Matthias Lenz, Arne Skerra   +7 more
wiley   +1 more source

Genetic tools for Sulfolobus spp.: vectors and first applications [PDF]

, 2018
Sulfolobus species belong to the best-studied archaeal organisms but have lacked powerful genetic methods. Recently, there has been considerable progress in the field of Sulfolobus genetics.
Berkner, Silvia, Lipps, Georg
core  

Display of the peroxiredoxin Bcp1 of Sulfolobus solfataricus on probiotic spores of Bacillus megaterium. [PDF]

, 2018
Bacterial spores displaying heterologous proteins have been proposed as a safe and efficient method for delivery of antigens and enzymes to animal mucosal surfaces.
Donadio, Giuliana, Fusco, FRANCESCA ANNA, Isticato, Rachele, Lanzilli, Mariamichela, Limauro, Danila, Ricca, Ezio, Sarcinelli, Carmen   +6 more
core   +1 more source

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Mass Spectrometry Reviews, Volume 44, Issue 3, Page 213-453, May/June 2025.
Abstract The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022.
David J. Harvey
wiley   +1 more source

Glycogen-bound polyphosphate kinase from the archaebacterium Sulfolobus acidocaldarius [PDF]

Journal of Bacteriology, 1989
Glycogen-bound polyphosphate kinase has been isolated from a crude extract of Sulfolobus acidocaldarius by isopycnic centrifugation in CsCl. Divalent cations (Mn2+ greater than Mg2+) stimulated the reaction. The enzyme does not require the presence of histones for its activity; it is inhibited strongly by phosphate and slightly by fluoride. The protein
R, Skórko, J, Osipiuk, K O, Stetter
openaire   +2 more sources