Results 41 to 50 of about 13,197 (261)
A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant Thermococcus kodakarensis has an altered cofactor preference for NAD+, as well as improved malate production compared to wildtype malate dehydrogenase.
Ravneet Mandair +2 more
doaj +1 more source
The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor
Transcript cleavage factors such as eukaryotic TFIIS assist the resumption of transcription following RNA pol II backtracking. Here the authors find that one of the Sulfolobus solfataricus TFIIS homolog—TFS4—has evolved into a potent RNA polymerase ...
Thomas Fouqueau +5 more
doaj +1 more source
A genetic study of SSV1, the prototypical fusellovirus.
Viruses of thermophilic Archaea are unique in both their structures and genomic sequences. The most widespread and arguably best studied are the lemon-shaped fuselloviruses.
Eric eIverson, Kenneth eStedman
doaj +1 more source
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba [PDF]
Archaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly ...
Ingledew, W John +3 more
core +1 more source
Background Exposure to nickel (Ni) and its chemical derivatives has been associated with severe health effects in human. On the contrary, poor knowledge has been acquired on target physiological processes or molecular mechanisms of this metal in model ...
Scaloni Andrea +6 more
doaj +1 more source
The Archaeal Elongation Factor EF-2 Induces the Release of aIF6 From 50S Ribosomal Subunit
The translation factor IF6 is a protein of about 25 kDa shared by the Archaea and the Eukarya but absent in Bacteria. It acts as a ribosome anti-association factor that binds to the large subunit preventing the joining to the small subunit.
Giada Lo Gullo +7 more
doaj +1 more source
ESCRT-III mediated cell division in Sulfolobus acidocaldarius - a reconstitution perspective [PDF]
In the framework of synthetic biology, it has become an intriguing question what would be the minimal representation of cell division machinery. Thus, it seems appropriate to compare how cell division is realized in different microorganisms. Inparticular,
Härtel, T., Schwille, P.
core +2 more sources
Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus [PDF]
Glucose dehydrogenase has been purified to homogeneity from cell extracts of the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme utilizes both NAD+ and NADP+ as coenzyme and catalyses the oxidation of several monosaccharides to the corresponding glyconic acid. Substrate specificity and oxidation rate depend on the coenzyme
GIARDINA, PAOLA +4 more
openaire +4 more sources
The S-layer is a proteinaceous envelope often found in bacterial and archaeal cells. Here, the authors use CRISPR-based technology to silence slaB, encoding the S-layer membrane anchor, to show that an intact S-layer is important for cell division and ...
Isabelle Anna Zink +5 more
doaj +1 more source
Rings in the Extreme: PCNA Interactions and Adaptations in the Archaea [PDF]
Biochemical and structural analysis of archaeal proteins has enabled us to gain great insight into many eukaryotic processes, simultaneously offering fascinating glimpses into the adaptation and evolution of proteins at the extremes of life. The archaeal
Bunting, KA, Winter, JA
core +2 more sources

