Results 141 to 150 of about 50,255 (242)

SUMO-1 modification regulates the protein stability of the large regulatory protein Rep78 of adeno associated virus type 2 (AAV-2)

Virology, 2004
The large Rep proteins Rep78 and Rep68 of the helper-dependent adeno associated virus type 2 (AAV-2) are essential for both site-specific integration of AAV DNA in the absence of helpervirus and productive AAV replication in the presence of helpervirus.
Weger, Stefan, Hammer, Eva, Heilbronn, Regine   +2 more
openaire   +2 more sources

The atomic structure of human dystrophin spectrin‐like repeat 24

Acta Crystallographica Section F, Volume 82, Issue 5, Page 184-193, May 2026.
The atomic structure of human dystrophin spectrin‐like repeat 24 was determined at 2.14 Å resolution.The structure of spectrin‐like repeat 24 of human dystrophin was determined at 2.5 Å effective resolution. The structure exhibits a three‐helix bundle fold, common to all spectrin‐repeat family members, and shares a high degree of homology with existing
Oakley Streeter, Ke Shi, Hannah Bui, Hideki Aihara, James M. Ervasti, Robert Evans III, Joseph M. Muretta   +6 more
wiley   +1 more source

Optimal 1TEL–target protein linker character is target protein‐dependent

Acta Crystallographica Section D, Volume 82, Issue 5, Page 516-532, May 2026.
In this study, we examine the effect of short to medium‐length flexible, semi‐flexible and rigid linkers on the crystallization of a DARPin or the TNK1 UBA domain fused to the 1TEL protein crystallization chaperone, demonstrating that while rigid linkers can impair crystallization and reduce diffraction quality, the ideal linker character remains ...
Maria J. Pedroza Romo, Alihikaua Keliiliki, Jacob C. Averett, Joseph F. Gonzalez, Ethan Noakes, Elijah W. Wilson, Conrad Smith, Blake Averett, Dalton Hansen, Riley Nickles, Miles Bradford, Sara Soleimani, Tobin Smith, Supeshala Nawarathnage, Prasadika Samarwickrama, Ariel Kelsch, Derick Bunn, Cameron Stewart, Wisdom Abiodun, Evan Tsubaki, Seth Brown, Tzanko I. Doukov, James D. Moody   +22 more
wiley   +1 more source

Opening closed inward rectifier potassium channel doors

British Journal of Pharmacology, Volume 183, Issue 10, Page 2197-2218, May 2026.
Inwardly rectifying potassium (KIR) channels are essential regulators of membrane potential in excitable and non‐excitable tissues. Although KIR channels exhibit a biophysical preference for potassium influx due to voltage‐dependent block of outward current by polyamines and Mg2+, under physiological conditions, they predominantly mediate K+ efflux ...
Anna Stary‐Weinzinger, Fabian Kaiser, Marcel A. G. van der Heyden, Saïd Bendahhou   +3 more
wiley   +1 more source

Probing the Cancer Mutational Landscape of KMT2 Regulatory Subunits

The FASEB Journal, Volume 40, Issue 7, 15 April 2026.
Pipeline to characterize the KMT2 cancer mutational landscape starts with querying genomic databases (top left) for mutations mapping to ASH2L, RbBP5, WDR5, and DPY‐30. Subsequent steps include the expression of these mutants in cell (bottom left), their systemic purification and biochemical characterization using isothermal titration calorimetry ...
Sabrina Grégoire, Sara Chow, Monika Joshi, Pamela Zhang, Aws Almir Ahmad, Ashley Janna, Veronique Tremblay, Marcelo Muñoz, Arvind Mer, Jean‐Francois Couture   +9 more
wiley   +1 more source

Elevating Haloperoxidase Expression in Escherichia coli through Fusion with a Formate Oxidase

ChemBioChem, Volume 27, Issue 7, 14 April 2026.
Fusing Aspergillus oryzae formate oxidase (AoFOx) to Curvularia inaequalis vanadium chloroperoxidase (CiVCPO) boosts soluble expression and enables formate‐driven, in situ H2O2 supply for halogenation. Screening linker/orientation variants delivered up to 9‐fold higher haloperoxidase activity in E.
Angelique Pothuizen, Jacob M. A. van Hengst, Ron Wever, Peter-Leon Hagedoorn, Frank Hollmann   +4 more
wiley   +1 more source

Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge's disease. [PDF]

, 2016
In this study, because excessive polycythemia is a predominant trait in some high-altitude dwellers (chronic mountain sickness [CMS] or Monge's disease) but not others living at the same altitude in the Andes, we took advantage of this human experiment ...
Appenzeller, Otto, Azad, Priti, Bafna, Vineet, Cabrales, Pedro J, Haddad, Gabriel G, Hsiao, Yu Hsin, Poulsen, Orit, Ronen, Roy, Zhao, Huiwen W, Zhou, Dan   +9 more
core  

Synthesis and characterization of different immunogenic viral nanoconstructs from rotavirus VP6 inner capsid protein

International Journal of Nanomedicine, 2014
Francesca Bugli,1 Valeria Caprettini,2 Margherita Cacaci,1 Cecilia Martini,1 Francesco Paroni Sterbini,1 Riccardo Torelli,1 Stefano Della Longa,3 Massimiliano Papi,4 Valentina Palmieri,4 Bruno Giardina,5 Brunella Posteraro,1 Maurizio Sanguinetti,1 ...
Bugli F, Caprettini V, Cacaci M, Martini C, Paroni Sterbini F, Torelli R, Della Longa S, Papi M, Palmieri V, Giardina B, Posteraro B, Sanguinetti M, Arcovito A   +12 more
doaj  

What have we learned from two-pore potassium channels? Their molecular configuration and function in the human heart [PDF]

, 2012
Two-pore domain potassium channels (K2P) control excitability, stabilize the resting membrane potential below firing threshold, and accelerate repolarisation in different cells.
Benyhe, Sándor, Boyett, Marc, Dobrzynski, Halina, Dézsi, László, Erdélyi, Lajos, Papp, Gyula, Szalontai, Balázs, Szüts, Viktória, Varró, András, Vágvölgyi, Csaba, Zhang, Henggui, Ötvös, Ferenc   +11 more
core  

Denaturing SUMO Immunoprecipitation From Mitotic Cells. [PDF]

Bio Protoc
Walker AK, Lanz AJ, Morris JR.
europepmc   +1 more source