The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme
Proceedings of the National Academy of Sciences, 1998 The ubiquitin-like protein SMT3 fromSaccharomyces cerevisiaeand SUMO-1, its mammalian homolog, can be covalently attached to other proteins posttranslationally. Conjugation of ubiquitin requires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes and proceeds via thioester-linked enzyme-ubiquitin intermediates. Herein we show that
S E, Schwarz +4 more
openaire +3 more sources
A Monoclonal Antibody Against the Human SUMO-1 Protein Obtained by Immunization with Recombinant Protein and CpG-DNA-liposome Complex [PDF]
Monoclonal Antibodies in Immunodiagnosis & Immunotherapy, 2013 Post-translational modification regulated by conjugation of a small ubiquitin-like modifier (SUMO) is involved in various cellular processes. In this study, we expressed and purified recombinant human SUMO-1 (hSUMO-1). BALB/c mice were immunized with a complex of hSUMO-1 protein and Lipoplex(O) to produce hSUMO-1-specific antibodies. Using conventional
Dongbum, Kim +6 more
openaire +2 more sources
A Modular and Programmable Cas13d Platform for RNA Single Nucleotide Variant Detection
Advanced Science, EarlyView.A scenario‐guided Cas13d platform enables configurable single nucleotide variant ribonucleic acid diagnostics through rule‐based guide design and ribonucleic acid binding domain engineering, supporting amplification‐free ultrasensitive detection and amplification‐coupled robust detection for accurate mutation classification in clinical tumor specimens.
Zeyu Wang +8 more
wiley +1 more source
Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications
mBio, 2019 Regulation of a variety of different cellular processes, including posttranslational modifications, is critical for the ability of many viruses to replicate efficiently within host cells.
Sook-Young Sohn, Patrick Hearing
doaj +1 more source
Transcription factor Sp3 as target for SUMOylation in vivo [PDF]
, 2005 A group of sequence-specific DNA-binding proteins related to the transcription factor Sp1 (specificity protein 1) has been implicated in the regulation of many different genes, since binding sites for these transcription factors (GC/GT boxes) are a ...
Renkawitz-Pohl, Renate (Prof. Dr.) +1 more
core +1 more source
Sensing and Filtering Environmental Fluctuations: The Case of Biomolecular Condensates in Plants
Advanced Science, EarlyView.The diversity of plant condensates reflects constraints of sessile organisms to coordinate postembryonic development with environmental adaptation. This review examines how plants employ condensates to integrate temperature, light, redox, and nutrient signals.
Panagiotis N. Moschou, Dorothee Staiger
wiley +1 more source
The C-terminus of p63 contains multiple regulatory elements with different functions [PDF]
, 2010 The transcription factor p63 is expressed as at least six different isoforms, of which two have been assigned critical biological roles within ectodermal development and skin stem cell biology on the one hand and supervision of the genetic stability of ...
Candi, Eleonora +8 more
core +1 more source
Synthetic Strategies for Activity‐Based Probes to Decode Ubiquitin‐Like Modifiers
Chemistry – A European Journal, EarlyView.ABSTRACT Ubiquitin‐like proteins (Ubls) such as SUMO, NEDD8, ISG15, URM1, UFM1, FAT10, ATG8/ATG12, and FUBI are essential regulators of cellular homeostasis, controlling processes from protein stability and trafficking to immune signaling and autophagy.
Saibal Chanda +5 more
wiley +1 more source
Modulation of inducible nitric oxide synthase expression by sumoylation
Journal of Neuroinflammation, 2009 Background In astrocytes, the inflammatory induction of Nitric Oxide Synthase type 2 (NOS2) is inhibited by noradrenaline (NA) at the transcriptional level however its effects on specific transcription factors are not fully known.
Feinstein Douglas L, Akar Candan A
doaj +1 more source
Androgen receptor acetylation governs trans activation and MEKK1-induced apoptosis without affecting in vitro sumoylation and trans-repression function [PDF]
, 2002 This work was supported by grants from the NIH (R01CA86072 to R.G.P. and R01CA72038-01 to S.A.W.F.) and The Susan Komen Breast Cancer Foundation (to R.G.P.). R.T.H. and E.J. were supported by the Medical Research Council. Y.-G.Y.
Chang, Chawnshang +13 more
core +1 more source