In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
Endothelial and smooth muscle cell interactions with a PCL-PU composite vascular scaffold with potential for bioactive release [PDF]
Paper discussing endothelial and smooth muscle cell interactions with a PCL-PU composite vascular scaffold with potential for bioactive ...
Black, Richard +2 more
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Rainfed cultivation with supplemental irrigation modelling on seed yield and oil of Coriandrum sativum L. using precision agriculture and GIS moisture mapping [PDF]
Agathos Filintas +2 more
openalex +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Combined treatment of biomatrices with nisin and pulsed electric fields as a potential decontamination method? [PDF]
Pulsed electric field (PEF) treatment has been shown to achieve bacterial inactivation in collagen gels whilst retaining the ability of the collagen to function as a biomaterial [1, 2].
Anderson, J.G. +4 more
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Lipid-based nutrient supplements: how can they combat child malnutrition? [PDF]
Kathryn Dewey and Mary Arimond discuss new research in PLOS Medicine that assesses the effect of blanket provision of ready-to-use supplementary food to children at high risk of malnutrition in Chad, and highlight some of the challenges of investigating ...
Arimond, Mary, Dewey, Kathryn G
core +1 more source
By dawn or dusk—how circadian timing rewrites bacterial infection outcomes
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo +2 more
wiley +1 more source
Organic Centre Wales Factsheet 22: Feeding to organic standards [PDF]
This factsheet states the general principles of feeding to organic standards. Issues surrounding the use of conventional feedstuffs, supplements and the feeding of young animals are also ...
Anon
core
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang +5 more
wiley +1 more source
Dietary Supplement Labeling: Cognitive Biases, Market Manipulation & Consumer Choice [PDF]
There exists increasing concern that the Dietary Supplements Health and Education Act (DSHEA) has proven ineffective. Much of the concern regards the disparity in legislative treatment between dietary supplements, foods, and pharmaceutical drugs. Namely,
McCann, Michael
core +1 more source

