Results 191 to 200 of about 29,778 (263)
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Studies of the respiration of synaptosomes
Biochemical Pharmacology, 1971Abstract Synaptosomes were prepared from guinea pig cerebral cortex and their respiration studied under different conditions. Their respiratory rate of 15 μl O 2 mg protein/hr in phosphate buffer containing glucose agrees with published values. This rate was not altered by the addition of K + , phenobarbitone, glutamic acid, glycine, γ-aminobutyric ...
D J, Balfour, J C, Gilbert
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Synaptosomes and cell separation
Neuroscience, 1976Publisher Summary When fractionating a complex tissue like brain, experiments should begin by separating it into its constituent cell classes. Any subcellular fraction prepared from brain is intrinsically heterogeneous as it is derived from several cell types.
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Subunit structure of synaptosomal tubulin
Brain Research, 1979The subunit structure of rat brain synaptosomal tubulin was examined by high resolution two-dimensional gel fractionation. Whole brain cytoplasmic tubulin consists of two groups of alpha subunits (alpha1 and alpha2), and a minimum of two beta subunits (beta1 and beta2).
C A, Marotta, P, Strocchi, J M, Gilbert
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Synaptosomal Transport Processes
1976Publisher Summary This chapter provides an overview of synaptosomal transport processes. The synaptosome is a fundamental in vitro preparation for studying the dynamic biochemical aspect of neurotransmission and neurosecretion. It qualifies, in many respects, as a functioning presynaptic entity with an impressive range of metabolic and transmission ...
G, Levi, M, Raiteri
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On the metabolism of ornithine in synaptosomal preparations
Neurochemical Research, 1982The present results show that ornithine is metabolized to glutamate by isolated synaptosomes from mouse cerebral cortex. Under the experimental conditions used the glutamate was channelled further to the tricarboxylic acid cycle, and to a lesser degree to GABA. The possible significance of these metabolic pathways are discussed.
S P, Lapinjoki +3 more
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Journal of Pharmacology and Experimental Therapeutics, 1969
We have examined the uptake of dl -H3-norepinephrine and H3-dopamine into homogenates from different regions of the rat brain. When the homogenates were centrifuged on continuous sucrose gradients, the H3-amine uptake was localized to fractions that were
J. Coyle, S. Snyder
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We have examined the uptake of dl -H3-norepinephrine and H3-dopamine into homogenates from different regions of the rat brain. When the homogenates were centrifuged on continuous sucrose gradients, the H3-amine uptake was localized to fractions that were
J. Coyle, S. Snyder
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Purification of Synaptosome Populations Using Fluorescence-Activated Synaptosome Sorting
2016For several decades, neurobiologists have used subcellular fractionation methods to analyze the molecular structure and some functional features of the cells in the central nervous system. Indeed, brain tissue contains a complex intermingled network of neuronal, glial, and vascular cells.
Luquet, Elisa +3 more
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Sulphated glycoproteins in synaptosomes
Neuroscience Letters, 1980Synaptosomes from sheep brain were incubated with Na2(35)SO4. After lysis at least twelve 35S-containing proteins were identified in the synaptosomal soluble fraction with molecular weights ranging between 13,000 and 150,000. Extraction of the particulate fraction with SDS/urea revealed a further eight [35S]proteins with 27,000-140,000 molecular ...
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Characterization of Melittin Effects in Synaptosomes
Neurochemical Research, 1997The effects of melittin at increasing concentrations on: [3H]GABA release from mouse brain synaptosomes; on the radioactivity released from [3H]arachidonic acid labeled synaptosomal membranes; on synaptosomes ultrastructure and on the leakage of the cytoplasmic marker, lactate-dehydrogenase (LDH) was investigated.
L, González +3 more
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