Results 71 to 80 of about 2,829,869 (398)

Machine learning approaches to predict the Plant-associated phenotype of Xanthomonas strains

BMC Genomics, 2021
Background The genus Xanthomonas has long been considered to consist predominantly of plant pathogens, but over the last decade there has been an increasing number of reports on non-pathogenic and endophytic members.
Dennie te Molder, Wasin Poncheewin, Peter J. Schaap, Jasper J. Koehorst   +3 more
doaj   +1 more source

Environment and synthetic biology. [PDF]

Synth Syst Biotechnol
Tang H, Song M.
europepmc   +4 more sources

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae

Microbial Cell Factories, 2021
Background Saccharomyces cerevisiae is an important synthetic biology chassis for microbial production of valuable molecules. Promoter engineering has been frequently applied to generate more synthetic promoters with a variety of defined characteristics ...
Jiliang Deng, Yanling Wu, Zhaohui Zheng, Nanzhu Chen, Xiaozhou Luo, Hongting Tang, Jay D. Keasling   +6 more
doaj   +1 more source

The intracellular domain of TLR2 is capable of high‐affinity Zn binding: possible outcomes for the receptor activation

FEBS Letters, EarlyView.
Toll‐like receptors (TLRs) are important in the innate immune system. This study explores the zinc‐binding ability of the TLR2 TIR domain (TLR2TIR). We found that TLR2TIR binds zinc with nanomolar affinity through its cysteine residues. Two of them, C673 and C713, are vital for receptor activation, indicating that zinc may play a role in initiating ...
Vladislav A. Lushpa, Cong Lin, Irina A. Talyzina, Marina V. Goncharuk, Eduard V. Bocharov, Alexander S. Arseniev, Xiaohui Wang, Sergey A. Goncharuk, Konstantin S. Mineev   +8 more
wiley   +1 more source

Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

Nature Communications, 2022
The yeast Saccharomyces cerevisiae is powerful for studying human G protein-coupled receptors as they can be coupled to its mating pathway. Here the authors engineer baker’s yeast to produce human sterols and show that vertebrate G protein coupled ...
Björn D. M. Bean, Colleen J. Mulvihill, Riddhiman K. Garge, Daniel R. Boutz, Olivier Rousseau, Brendan M. Floyd, William Cheney, Elizabeth C. Gardner, Andrew D. Ellington, Edward M. Marcotte, Jimmy D. Gollihar, Malcolm Whiteway, Vincent J. J. Martin   +12 more
doaj   +1 more source

Multicriteria global optimization for biocircuit design [PDF]

, 2014
One of the challenges in Synthetic Biology is to design circuits with increasing levels of complexity. While circuits in Biology are complex and subject to natural tradeoffs, most synthetic circuits are simple in terms of the number of regulatory regions,
Banga, Julio R., Otero-Muras, Irene
core   +3 more sources

The power of microRNA regulation—insights into immunity and metabolism

FEBS Letters, EarlyView.
MicroRNAs are emerging as crucial regulators at the intersection of metabolism and immunity. This review examines how miRNAs coordinate glucose and lipid metabolism while simultaneously modulating T‐cell development and immune responses. Moreover, it highlights how cutting‐edge artificial intelligence applications can identify miRNA biomarkers ...
Stefania Oliveto, Nicola Manfrini, Stefano Biffo   +2 more
wiley   +1 more source

Creation of a eukaryotic multiplexed site-specific inversion system and its application for metabolic engineering

Nature Communications
The site-specific recombination system is a versatile tool in genome engineering, enabling controlled DNA inversion or deletion at specific sites to generate genetic diversity.
Jieyi Li, Simiao Gong, Yuan Ma, Peiyan Han, Nan Wang, Zongheng Fu, Xinyi Zhang, Xinyang Huang, Tianyu Yang, Hanze Tong, Guang-Rong Zhao, Yi Wu, Ying-Jin Yuan   +12 more
doaj   +1 more source

Coiled-coil heterodimers with increased stability for cellular regulation and sensing SARS-CoV-2 spike protein-mediated cell fusion

Scientific Reports, 2021
Coiled-coil (CC) dimer-forming peptides are attractive designable modules for mediating protein association. Highly stable CCs are desired for biological activity regulation and assay.
Tjaša Plaper, Jana Aupič, Petra Dekleva, Fabio Lapenta, Mateja Manček Keber, Roman Jerala, Mojca Benčina   +6 more
doaj   +1 more source