Results 41 to 50 of about 3,003,336 (196)

Locus specific engineering of tandem DNA repeats in the genome of Saccharomyces cerevisiae using CRISPR/Cas9 and overlapping oligonucleotides

Scientific Reports, 2018
DNA repeats constitute a large part of genomes of multicellular eucaryotes. For a longtime considered as junk DNA, their role in genome organization and tuning of gene expression is being increasingly documented.
Astrid Lancrey, Alexandra Joubert, Jean-Baptiste Boulé   +2 more
doaj   +1 more source

DCs facilitate B cell responses against microbial DNA via DC-SIGN.

PLoS ONE, 2017
Microbial DNA is highly immunostimulatory and is sensed by endosomal pattern recognition receptors after release from internalized microbes. It is unclear how extracellular DNA released from dead microbes is delivered to endosomal PRRs to induce immune ...
Joris K Sprokholt, Marieke H Heineke, Tanja M Kaptein, John L van Hamme, Teunis B H Geijtenbeek   +4 more
doaj   +1 more source

Unconventional Lysine-Type Lipid Assemblies Enable Efficient Antisense Oligonucleotide Delivery with Distinct Structural Features

Pharmaceutics
Background/Objectives: Antisense oligonucleotides (ASOs) hold great therapeutic potential due to their precise ability to modulate gene expression, particularly for treating genetic and neurological disorders.
Jieyan He, Whitney Shatz-Binder, Alexandra Robles, Nanzhi Zang, Wei Jia, Sakura Sahai, Matthew C. Johnson, Jing Li, Chun-Wan Yen, Shinji Takeoka   +9 more
doaj   +1 more source

Class I histone deacetylase inhibition is synthetic lethal with BRCA1 deficiency in breast cancer cells

Acta Pharmaceutica Sinica B, 2020
Breast cancer susceptibility gene 1 (BRCA1) is a tumor suppressor gene, which is frequently mutated in breast and ovarian cancers. BRCA1 plays a key role in the homologous recombination directed DNA repair, allowing its deficiency to act as a therapeutic
Baoyuan Zhang, Junfang Lyu, Eun Ju Yang, Yifan Liu, Changjie Wu, Lakhansing Pardeshi, Kaeling Tan, Qiang Chen, Xiaoling Xu, Chu-Xia Deng, Joong Sup Shim   +10 more
doaj   +1 more source

Shuffling of DNA Cassettes in a Synthetic Integron [PDF]

, 2013
The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle ...
Bikard, David, Mazel, Didier
openaire   +3 more sources

Processing DNA Storage through Programmable Assembly in a Droplet‐Based Fluidics System

Advanced Science, 2023
DNA can be used to store digital data, and synthetic short‐sequence DNA pools are developed to store high quantities of digital data. However, synthetic DNA data cannot be actively processed in DNA pools.
Minsang Yu, Doyeon Lim, Jungwoo Kim, Youngjun Song   +3 more
doaj   +1 more source

Autonomous assembly of synthetic oligonucleotides built from an expanded DNA alphabet. Total synthesis of a gene encoding kanamycin resistance

Beilstein Journal of Organic Chemistry, 2014
Background: Many synthetic biologists seek to increase the degree of autonomy in the assembly of long DNA (L-DNA) constructs from short synthetic DNA fragments, which are today quite inexpensive because of automated solid-phase synthesis.
Kristen K. Merritt, Kevin M. Bradley, Daniel Hutter, Mariko F. Matsuura, Diane J. Rowold, Steven A. Benner   +5 more
doaj   +1 more source

DNA Photonics – Photoinduced Electron Transfer in Synthetic DNA-Donor–Acceptor Systems

CHIMIA, 2007
Recent experimental and theoretical results have demonstrated that structural dynamics are critical for a comprehensive mechanistic understanding of DNA charge transfer (CT).
Torsten Fiebig, Hans-Achim Wagenknecht
doaj   +1 more source

Transcriptional Regulation and Combinatorial Genetic Logic in Synthetic Bacterial Circuits [PDF]

, 2008
We engineered several synthetic regulatory circuits to study transcriptional regulation in bacteria. We developed a new technique for DNA construction, built and characterized in vivo a library of genetic logic gates, examined the role of genetic noise ...
Cox, Robert Sidney, III
core   +1 more source

DNA-Origami surface patterning through polymer conjugation [PDF]

, 2022
The combination of DNA-origami and synthetic polymers paves the way to a new class of structurally precise biohybrid nanomaterials for diverse applications.
David, Ng, Nico, Alleva, Pia , Winterwerber, Colette J., Whitfield, Tanja, Weil, Pia Winterwerber, Nico Alleva, Tanja Weil, David Ng, Colette J. Whitfield   +9 more
core   +2 more sources