Results 51 to 60 of about 2,867,164 (299)
Transcriptional Regulation and Combinatorial Genetic Logic in Synthetic Bacterial Circuits [PDF]
, 2008 We engineered several synthetic regulatory circuits to study transcriptional regulation in bacteria. We developed a new technique for DNA construction, built and characterized in vivo a library of genetic logic gates, examined the role of genetic noise ...
Cox, Robert Sidney, III
core +1 more source
Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening. [PDF]
PLoS ONE, 2016 To date, lentiviral-based CRISPR-Cas9 screens have largely been conducted in pooled format. However, numerous assays are not amenable to pooled approaches, and lentiviral screening in arrayed format presents many challenges.
Jenille Tan, Scott E Martin
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Bioengineered, 2013 With world wide data predicted to exceed 40 trillion gigabytes by 2020, big data storage is a very real and escalating problem. Herein, we discuss the utility of synthetic DNA as a robust and eco-friendly archival data storage solution of the future.
O’ Driscoll, Aisling, Sleator, Roy D.
openaire +2 more sources
DNA-Based asymmetric catalysis as a synthetic tool [PDF]
, 2012 De helische structuur van DNA heeft altijd al aantrekkingskracht gehad op chemici. In onze groep hebben we DNA gebruikt om een enantioselectieve katalysator te ontwikkelen.
Megens, Rikkert Pepijn, +1 more
core +10 more sources
DNA-based biosensor platforms for the detection of TP53 mutation [PDF]
, 2012 A DNA-based assay for the detection of one-point mutation in TP53 gene, responsible for lung cancer, was developed using a surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM) biosensor systems.
Altintas, Zeynep +3 more
core +1 more source
Importance of the 5′ regulatory region to bacterial synthetic biology applications
Microbial Biotechnology, 2021 Summary The field of synthetic biology is evolving at a fast pace. It is advancing beyond single‐gene alterations in single hosts to the logical design of complex circuits and the development of integrated synthetic genomes.
Lisa Tietze, Rahmi Lale
doaj +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Lego DNA assembling, a simple in vitro method for constructing DNA molecule [PDF]
, 2010 Digestion-ligation based and recombination based methods for constructing recombinant DNA are the basic techniques in molecular biology and thus built one of the foundations for the modern life sciences. Here we describe a new strategy that can radically
Cong Wang +7 more
core
Correction of the neuropathogenic human apolipoprotein E4 (APOE4) gene to APOE3 in vitro using synthetic RNA/DNA oligonucleotides (chimeraplasts) [PDF]
, 2005 Apolipoprotein E (apoE) is a multifunctional circulating 34-kDa protein, whose gene encodes single-nucleotide polymorphisms linked to several neurodegenerative diseases.
Owen, JS +7 more
core +1 more source