Bioengineered, 2013 With world wide data predicted to exceed 40 trillion gigabytes by 2020, big data storage is a very real and escalating problem. Herein, we discuss the utility of synthetic DNA as a robust and eco-friendly archival data storage solution of the future.
O’ Driscoll, Aisling, Sleator, Roy D.
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Lego DNA assembling, a simple in vitro method for constructing DNA molecule [PDF]
, 2010 Digestion-ligation based and recombination based methods for constructing recombinant DNA are the basic techniques in molecular biology and thus built one of the foundations for the modern life sciences. Here we describe a new strategy that can radically
Cong Wang +7 more
core
Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening. [PDF]
PLoS ONE, 2016 To date, lentiviral-based CRISPR-Cas9 screens have largely been conducted in pooled format. However, numerous assays are not amenable to pooled approaches, and lentiviral screening in arrayed format presents many challenges.
Jenille Tan, Scott E Martin
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From DNA nanotechnology to synthetic biology [PDF]
HFSP Journal, 2008 Attempts to construct artificial systems from biological molecules such as DNA and RNA by self-assembly are compatible with the recent development of synthetic biology. Genetic mechanisms can be used to produce or control artificial structures made from DNA and RNA, and these structures can in turn be used as artificial gene regulatory elements, in ...
Ralf, Jungmann +2 more
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Correction of the neuropathogenic human apolipoprotein E4 (APOE4) gene to APOE3 in vitro using synthetic RNA/DNA oligonucleotides (chimeraplasts) [PDF]
, 2005 Apolipoprotein E (apoE) is a multifunctional circulating 34-kDa protein, whose gene encodes single-nucleotide polymorphisms linked to several neurodegenerative diseases.
Owen, JS +7 more
core +1 more source
Synthetic Lethality between DNA Polymerase Epsilon and RTEL1 in Metazoan DNA Replication
Cell Reports, 2020 Summary: Genome stability requires coordination of DNA replication origin activation and replication fork progression. RTEL1 is a regulator of homologous recombination (HR) implicated in meiotic cross-over control and DNA repair in C. elegans.
Roberto Bellelli +5 more
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Importance of the 5′ regulatory region to bacterial synthetic biology applications
Microbial Biotechnology, 2021 Summary The field of synthetic biology is evolving at a fast pace. It is advancing beyond single‐gene alterations in single hosts to the logical design of complex circuits and the development of integrated synthetic genomes.
Lisa Tietze, Rahmi Lale
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Circular single-stranded DNA as switchable vector for gene expression in mammalian cells
Nature Communications, 2023 Synthetic gene networks in mammalian cells are currently limited to either protein-based transcription factors or RNA-based regulators. Here, we demonstrate a regulatory approach based on circular single-stranded DNA (Css DNA), which can be used as an ...
Linlin Tang +10 more
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OVERFRAG: An overlapping DNA fragments generator for molecular cloning and synthetic biology
Data in Brief, 2019 DNA synthesis and homologous recombination can be used to simplify molecular cloning and to make synthetic biology easily accessible (M.J. Czar et al., 2009). However, the design of overlapping DNA fragments to construct large molecules is time-consuming
Jose Ribamar Ferreira-Junior +1 more
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HIV-1 Env DNA vaccine plus protein boost delivered by EP expands B- and T-cell responses and neutralizing phenotype in vivo. [PDF]
PLoS ONE, 2013 An effective HIV vaccine will most likely require the induction of strong T-cell responses, broadly neutralizing antibodies (bNAbs), and the elicitation of antibody-dependent cellular cytotoxicity (ADCC).
Kar Muthumani +13 more
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