Methanol Dehydrogenases as a Key Biocatalysts for Synthetic Methylotrophy [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 One-carbon (C1) chemicals are potential building blocks for cheap and sustainable re-sources such as methane, methanol, formaldehyde, formate, carbon monoxide, and more.
Thien-Kim Le +5 more
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Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy [PDF]
Microorganisms, 2022 Formaldehyde metabolism is prevalent in all organisms, where the accumulation of formaldehyde can be prevented through the activity of dissimilation pathways.
Vivien Jessica Klein +4 more
doaj +6 more sources
Synthetic C1 metabolism in Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway [PDF]
mBioFormate and methanol are promising alternatives to sugar-based feedstocks for biotechnological applications. These one-carbon (C1) substrates can be sustainably produced from CO2 and renewable electricity and assimilated by both native and engineered ...
Justine Turlin +5 more
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Toward Methanol-Based Biomanufacturing: Emerging Strategies for Engineering Synthetic Methylotrophy in Saccharomyces cerevisiae. [PDF]
ACS Synth Biol, 2022 The global expansion of biomanufacturing is currently limited by the availability of sugar-based microbial feedstocks, which require farmland for cultivation and therefore cannot support large increases in production without impacting the human food supply.
Kelso PA +4 more
europepmc +3 more sources
Synthetic methylotrophic yeasts for the sustainable fuel and chemical production [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Global energy-related emissions, in particular carbon dioxide, are rapidly increasing. Without immediate and strong reductions across all sectors, limiting global warming to 1.5 °C and thus mitigating climate change is beyond reach.
Vanessa Wegat +2 more
doaj +2 more sources
Development of a formaldehyde biosensor with application to synthetic methylotrophy. [PDF]
Biotechnol Bioeng, 2018 Formaldehyde is a prevalent environmental toxin and a key intermediate in single carbon metabolism. The ability to monitor formaldehyde concentration is, therefore, of interest for both environmental monitoring and for metabolic engineering of native and synthetic methylotrophs, but current methods suffer from low sensitivity, complex workflows, or ...
Woolston BM +4 more
europepmc +5 more sources
From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Engineering microorganisms to grow on alternative feedstocks is crucial not just because of the indisputable biotechnological applications but also to deepen our understanding of microbial metabolism.
Camille Peiro +3 more
doaj +2 more sources
Increasing lysine level improved methanol assimilation toward butyric acid production in Butyribacterium methylotrophicum [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Methanol, a promising non-food fermentation substrate, has gained increasing interest as an alternative feedstock to sugars for the bio-based production of value-added chemicals.
Jing Wang +7 more
doaj +2 more sources
Engineering and adaptive laboratory evolution of Escherichia coli for improving methanol utilization based on a hybrid methanol assimilation pathway [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Engineering Escherichia coli for efficient methanol assimilation is important for developing methanol as an emerging next-generation feedstock for industrial biotechnology. While recent attempts to engineer E.
Qing Sun +6 more
doaj +2 more sources
Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy [PDF]
Nature CommunicationsSynthetic methylotrophy offers opportunities for sustainable chemical and biofuel production. While recently established methylotrophic E. coli can grow on methanol, undesirable formate accumulation occurs during growth and bioproduction.
Philipp Keller +10 more
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