Improving the Methanol Tolerance of an Escherichia coli Methylotroph via Adaptive Laboratory Evolution Enhances Synthetic Methanol Utilization [PDF]
Frontiers in Microbiology, 2021 There is great interest in developing synthetic methylotrophs that harbor methane and methanol utilization pathways in heterologous hosts such as Escherichia coli for industrial bioconversion of one-carbon compounds.
R. Kyle Bennett +8 more
doaj +2 more sources
Leveraging substrate flexibility and product selectivity of acetogens in two-stage systems for chemical production [PDF]
Microbial Biotechnology, Volume 16, Issue 2, Page 218-237, February 2023., 2022 Carbon dioxide (CO2 ) stands out as sustainable feedstock for developing a circular carbon economy whose energy supply could be obtained by boosting the production of clean hydrogen from renewable electricity.
Bernacchi, Sebastien +5 more
core +2 more sources
Responses to organic pollutants in the tropical Pacific and subtropical Atlantic Oceans by pelagic marine bacteria [PDF]
Frontiers in Environmental Science, 2023 Background and chronic pollution by organic pollutants (OPs) is a widespread threat in the oceans with still uncharacterized effects on marine ecosystems and the modulation of major biogeochemical cycles.
Maria Vila-Costa +7 more
doaj +3 more sources
Scanning the active center of formolase to identify key residues for enhanced C1 to C3 bioconversion [PDF]
Bioresources and BioprocessingBackground Formolase (FLS) is a computationally designed enzyme that catalyzes the carboligation of two or three C1 formaldehyde molecules into C2 glycolaldehyde or C3 dihydroxyacetone (DHA). FLS lays the foundation for several artificial carbon fixation
Guimin Cheng +7 more
doaj +2 more sources
Synthetic Methylotrophy in Yeasts: Towards a Circular Bioeconomy [PDF]
Trends in Biotechnology, 2021 Mitigating climate change is a key driver for the development of sustainable and CO2-neutral production processes. In this regard, connecting carbon capture and utilization processes to derive microbial C1 fermentation substrates from CO2 is highly promising. This strategy uses methylotrophic microbes to unlock next-generation processes, converting CO2-
Jonathan Thomas, Fabarius +3 more
openaire +2 more sources
Identification and characterization of a novel formaldehyde dehydrogenase in Bacillus subtilis. [PDF]
Appl Environ MicrobiolFormaldehyde is a known toxic compound, and functional formaldehyde detoxification is crucial for the survival of all living cells. Such detoxification systems are of particular importance for methylotrophic microorganisms that rely on formaldehyde as a ...
Klein VJ +5 more
europepmc +3 more sources
Rare earth elements in biology: From biochemical curiosity to solutions for extractive industries. [PDF]
Microb BiotechnolTo meet society's demands for technologies that use rare earth elements (including those that allow a decarbonized society), we need to increase the volume and efficiency of extraction by mining and recycling. Microbial rare earth biochemistry offers numerous enabling biotechnologies to achieve this. Abstract Rare earth elements (REEs) are critical for
Rocha RA, Alexandrov K, Scott C.
europepmc +2 more sources
Industrial applicability of enzymatic and whole-cell processes for the utilization of C1 building blocks. [PDF]
Nat CommunChemicals produced through enzymatic reactions play a key role in the transition from a linear petrol-dependent to a circular bioeconomy. One promising approach is the conversion of single carbon (C1) molecules by biocatalysts to value-added products ...
Barone GD +11 more
europepmc +3 more sources
Lanthanide-Controlled Protein Switches: Development and In Vitro and In Vivo Applications. [PDF]
Angew Chem Int Ed EnglEngineering artificial allosteric protein switches controlled by rare earth elements allows the development of assays, construction of bioelectronic devices, and REE‐controlled microorganisms. Abstract Lanthanides, which are part of the rare earth elements group have numerous applications in electronics, medicine and energy storage.
Guo Z +9 more
europepmc +3 more sources
The cofactor challenge in synthetic methylotrophy: bioengineering and industrial applications
Current Opinion in Biotechnology, 2023 Methanol is a promising feedstock for industrial bioproduction: it can be produced renewably and has high solubility and limited microbial toxicity. One of the key challenges for its bio-industrial application is the first enzymatic oxidation step to formaldehyde.
Krüsemann, Jan L. +4 more
openaire +4 more sources