Results 21 to 30 of about 212,422 (259)

The Average IFN-γ Secreting Capacity of Specific CD8+ T Cells Is Compromised While Increasing Copies of a Single T Cell Epitope Encoded by DNA Vaccine

Clinical and Developmental Immunology, 2012
Previous studies suggested that both the frequency and the mean fluorescence intensity (MFI) of cytokine secreting T cells could be of great value for immunogenicity evaluation of a vaccine.
Yanmin Wan, Jing Wang, Haizhu Zhou, Zhidong Hu, Xiaonan Ren, Jianqing Xu   +5 more
doaj   +1 more source

Discordance Between the Predicted Versus the Actually Recognized CD8+ T Cell Epitopes of HCMV pp65 Antigen and Aleatory Epitope Dominance

Frontiers in Immunology, 2021
CD8+ T cell immune monitoring aims at measuring the size and functions of antigen-specific CD8+ T cell populations, thereby providing insights into cell-mediated immunity operational in a test subject.
Alexander A. Lehmann, Ting Zhang, Pedro A. Reche, Paul V. Lehmann   +3 more
doaj   +1 more source

B Epitope Multiplicity and B/T Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines

Clinical and Developmental Immunology, 2013
Synthetic peptides incorporating protective B- and T-cell epitopes are candidates for new safer foot-and-mouth disease (FMD) vaccines. We have reported that dendrimeric peptides including four copies of a B-cell epitope (VP1 136 to 154) linked to a T ...
Esther Blanco, Carolina Cubillos, Noelia Moreno, Juan Bárcena, Beatriz G. de la Torre, David Andreu, Francisco Sobrino   +6 more
doaj   +1 more source

Novel H7N9 influenza immunogen design enhances mobilization of seasonal influenza T cell memory in H3N2 pre-immune mice

Human Vaccines & Immunotherapeutics, 2022
Strategies that improve influenza vaccine immunogenicity are critical for the development of vaccines for pandemic preparedness. Hemagglutinin (HA)-specific CD4+ T cell epitopes support protective B cell responses against seasonal influenza.
Leonard Moise, Lauren M. Meyers, Hyesun Jang, Mayara Grizotte-Lake, Christine M. Boyle, Bethany McGonnigal, Pan Ge, Ted M. Ross, Anne S. De Groot   +8 more
doaj   +1 more source

Tracking epitope-specific T cells [PDF]

Nature Protocols, 2009
The tracking of antigen-specific T cells in vivo is a useful approach for the study of the adaptive immune response. This protocol describes how populations of T cells specific for a given peptide-major histocompatibility complex (pMHC) epitope can be tracked based solely on T-cell receptor (TCR) specificity as opposed to other indirect methods based ...
James J, Moon, H Hamlet, Chu, Jason, Hataye, Antonio J, Pagán, Marion, Pepper, James B, McLachlan, Traci, Zell, Marc K, Jenkins   +7 more
openaire   +2 more sources

Systematic profiling of SARS-CoV-2 structural protein-specific T cell epitopes in Omicron infections following inactivated vaccination

iScience
Summary: Comprehensive identification of T cell epitopes is crucial for understanding SARS-CoV-2-specific T cell immunity and guiding vaccine development.
Zhiqing Li, Mengmeng Cui, Jian Wu, Tianju Hu, Junyan Dan, Xiaosu Chen, Qicong Shen, Jin Hou, Zhongfang Wang, Yizhi Yu, Shuxun Liu   +10 more
doaj   +1 more source

Highly sensitive live-cell imaging-based cytotoxicity assay enables functional validation of rare epitope-specific CTLs

Frontiers in Immunology
Many immunotherapeutic approaches aim to induce epitope-specific T-cell cytotoxicity. However, the identification—and especially the functional validation—of suitable epitopes by in vitro cytotoxicity assays can be challenging, particularly when the ...
Kathrin Wellach, Kathrin Wellach, Kathrin Wellach, Angelika B. Riemer, Angelika B. Riemer   +4 more
doaj   +1 more source

Individual Epitope-Specific CD8⁺ T Cell Immune Responses Are Shaped Differently during Chronic Viral Infection

Pathogens, 2023
A hallmark in chronic viral infections are exhausted antigen-specific CD8+ T cell responses and the inability of the immune system to eliminate the virus.
Sebastian Klein, Jasmin Mischke, Finn Beruldsen, Immo Prinz, Dinler A. Antunes, Markus Cornberg, Anke R. M. Kraft   +6 more
doaj   +1 more source

Predicting T cell receptor functionality against mutant epitopes

Cell Genomics
Summary: Cancer cells and pathogens can evade T cell receptors (TCRs) via mutations in immunogenic epitopes. TCR cross-reactivity (i.e., recognition of multiple epitopes with sequence similarities) can counteract such escape but may cause severe side ...
Felix Drost, Emilio Dorigatti, Adrian Straub, Philipp Hilgendorf, Karolin I. Wagner, Kersten Heyer, Marta López Montes, Bernd Bischl, Dirk H. Busch, Kilian Schober, Benjamin Schubert   +10 more
doaj   +1 more source

FRED—a framework for T-cell epitope detection [PDF]

Bioinformatics, 2009
Abstract Summary: Over the last decade, immunoinformatics has made significant progress. Computational approaches, in particular the prediction of T-cell epitopes using machine learning methods, are at the core of modern vaccine design.
Magdalena Feldhahn, Pierre Dönnes, Philipp Thiel, Oliver Kohlbacher   +3 more
openaire   +2 more sources