Results 131 to 140 of about 28,279,407 (312)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Exploring options for the transfer of technology from the Canada-Ontario Agricultural Green Plan: Final report of the Technology Transder Committee

, 1997
The Green Plan Technology Transfer Committee (TTC) was established to deal with concerns related to the transfer of technology and information resulting from the Canada-Ontario Green Plan.
Technology Transfer Committee of the Canada-Ontario Agricultural Green Plan
core  

Teacher change in a developing curriculum area: the example of trade teachers in the New Zealand technology curriculum [PDF]

, 2000
Technology education for all students is a relatively new phenomenon in national and international curricula. Although New Zealand has a long history of technical education in the senior primary and secondary school, a curriculum for technology education
Jones, Alister, Alister Jones (7152164), Louis Ewington (7152167), Ewington, Louis   +3 more
core  

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Gas Vesicle-Assisted Ultrasound Imaging for Effective Anti-Tumour CAR-T Cell Immunotherapy Efficacy in Mice Model

International Journal of Nanomedicine
Yizhen Huang,1,2,* Zhao Yin,3,* Renhao Xu,2,* Wenyi Zheng,2 Qing Zhang,3 Meijun Zhou,2 Zhili Xu,2 Yanni He,2,4 Shuang Liu,3,4 Hongmei Liu1,2,4 1The Second School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, People’s ...
Huang Y, Yin Z, Xu R, Zheng W, Zhang Q, Zhou M, Xu Z, He Y, Liu S, Liu H   +9 more
doaj  

When the High Road Becomes the Low Road: The Limits of High-Technology Competition in Finland

, 2013
Atlanta Conference on Science and Innovation Policy 2013S&T PoliciesThis paper questions the degree to which high-technology growth represents a “high road” to growth by highlighting the economic and political risks associated with technology-oriented ...
Ornston, Darius
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