A study on the immobilization of pepsin on various nanomaterials (graphene oxide, boron nitride, and gold nanoparticles) was conducted. The immobilized pepsin was used to generate bioactive peptides, which were then evaluated for their antioxidant properties.
Annie N. Alves +8 more
wiley +1 more source
Methods for the Thermal Stabilization of α-L-Rhamnosidase and Inactivation of β-Glucosidase in the Naringinase Complex from <i>Aspergillus niger</i>. [PDF]
Bodakowska-Boczniewicz J, Garncarek Z.
europepmc +1 more source
The study presents an optimized, reproducible protoplast transfection method for Geobacillus stearothermophilus. The method enables efficient uptake of native and fully synthetic TP‐84 bacteriophage genomes, their “rebooting” in vivo and production of infectious phage particles.
Ireneusz Sobolewski +4 more
wiley +1 more source
Bst DNA Polymerase: Structure, Properties and Engineering Strategies in LAMP. [PDF]
Tikhonova E, Popinako A, Sazonov A.
europepmc +1 more source
The Lord of the Rings: Cysteine bonds crosslink the tail of siphovirus
Abstract Long, non‐contractile tails composed of helical hexameric protein repeats that assemble into continuous tubular structures characterize siphoviruses. The siphovirus tail tube protein gp39 contains two cysteine residues per monomer. Cryo‐electron microscopy revealed that these cysteines are oriented toward the interface between the rings, which
Simona Povilonienė +9 more
wiley +1 more source
Reductive Methylation: An Alternative to Lysine → Arginine Mutagenesis. [PDF]
Molina OJ +4 more
europepmc +1 more source
Conserved Asp233–Asp246 hydrogen bond modulates active site dynamics in class A β‐lactamases
Abstract Rigid enzymes catalyze chemical reactions by stabilizing the transition state through a specific conformation. Catalytic residues are precisely positioned, and enzyme dynamics are kept to a minimum. It is thought that conserved residues around the active site (second‐shell residues) play a crucial role in positioning catalytic residues. Asp233
Cristina V. Lopez‐Gallego +3 more
wiley +1 more source
Molecular Adaptations of Thermophilic Proteins-From Mechanistic Understanding to Machine Learning Approaches. [PDF]
Arunachalam S, Mm B, Gnanasekaran R.
europepmc +1 more source
Modular Metalloprotein Nanowires With Semiconductor‐Scale Conductivity and Tunable Redox Behavior
Conductive protein nanowires are built by aligning three different metalloproteins along an ultrastable filament scaffold. The resulting micrometer‐length nanowires reach conductivities of 1.75 S cm−1. Redox behavior is set by the metalloprotein chosen, while conductivity is governed by the spacing the scaffold imposes, making both independently ...
Nga T. Lam +3 more
wiley +1 more source
Engineering and Application of a Thermostable MHETase for PET Depolymerization. [PDF]
Murphy NP +17 more
europepmc +1 more source

