Results 41 to 50 of about 68,203 (335)

Enhancing thermal tolerance of Aspergillus niger PhyA phytase directed by structural comparison and computational simulation

open access: yesBMC Biotechnology, 2018
Background Phytase supplied in feeds for monogastric animals is important for improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability to withstand transient high ...
Nanyu Han   +7 more
doaj   +1 more source

Thermostability profiling of MHC-bound peptides: a new dimension in immunopeptidomics and aid for immunotherapy design

open access: yesNature Communications, 2020
Thermostability of the peptide-MHC interaction is important for immunogenicity. Here the authors present a mass spectrometry method to measure thermostability among thousands of peptide-MHC complexes in parallel and a trained artificial neural network to
Emma C. Jappe   +9 more
doaj   +1 more source

Oxyalkylated alcohols phthalates [PDF]

open access: yesНанотехнологии в строительстве, 2019
Polyvinyl chloride (PVC) is used to obtain a wide range of materials for various purposes. High demand of PVC is explained by possibility to modify polyvinyl chloride and produce broad range of materials with improved properties as well as to have cost
Albina R. Maskova   +4 more
doaj   +1 more source

Cellulase immobilization on superparamagnetic nanoparticles for reuse in cellulosic biomass conversion [PDF]

open access: yes, 2016
Current cellulosic biomass hydrolysis is based on the one-time use of cellulases. Cellulases immobilized on magnetic nanocarriers offer the advantages of magnetic separation and repeated use for continuous hydrolysis. Most immobilization methods focus on
Fernando Segato   +5 more
core   +3 more sources

Improving the Stability of Protein–Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
The protein–protein interaction assay is a key technology in various fields, being applicable in drug screening as well as in diagnosis and inspection, wherein the stability of assays is important.
Yuki Ohmuro-Matsuyama   +6 more
doaj   +1 more source

Stabilization versus flexibility: Detergent-dependent trade-offs in neurotensin receptor 1 GPCR ensembles. [PDF]

open access: yesProtein Sci
Abstract Detergents provide essential membrane‐mimetic environments for studying G protein‐coupled receptors (GPCRs), but their molecular impact on receptor energetics remains incompletely understood. We combined ligand binding, thermostability measurements, and atomistic molecular dynamics to dissect detergent‐ versus ligand‐driven stabilization in a ...
Bower JB   +6 more
europepmc   +2 more sources

Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications

open access: yesFEBS Open Bio, EarlyView.
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley   +1 more source

Overproduction of Tk-Subtilisin and Elucidation of Its Structural Thermostability Based on Molecular Dynamics Studies [PDF]

open access: yesBrazilian Archives of Biology and Technology
Background: Subtilisin from Thermococcus kodakaraensis (Tk-subtilisin) is an ideal candidate for many industrial and research processes due to its unique features. Methods: In this study, the enzyme is overproduced in E.
Nouman Rasool, Naeem Rashid
doaj   +1 more source

Improving Thermostability of Chimeric Enzymes Generated by Domain Shuffling Between Two Different Original Glucoamylases

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
In order to improve enzymatic properties of glucoamylases, six recombinant genes GA1–GA6 were created by domain shuffling of glucoamylase genes GAA1 from Aspergillus niger Ld418AI and GATE from Talaromyces emersonii Ld418 TE using overlap extension PCR ...
Zhongxiu Chen   +15 more
doaj   +1 more source

Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors. [PDF]

open access: yes, 2013
Vesicular stomatitis virus G glycoprotein (VSV-G) is the most widely used envelope protein for retroviral and lentiviral vector pseudotyping; however, serum inactivation of VSV-G pseudotyped vectors is a significant challenge for in vivo gene delivery ...
Hwang, B-Y, Schaffer, DV
core  

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