Results 61 to 70 of about 60,226 (297)

GmPGL1, a Thiamine Thiazole Synthase, Is Required for the Biosynthesis of Thiamine in Soybean

Frontiers in Plant Science, 2019
Thiamine is an essential cofactor in several enzymatic reactions for all living organisms. Animals cannot synthesize thiamine and depend on their diet.
Xingxing Feng, Xingxing Feng, Suxin Yang, Kuanqiang Tang, Kuanqiang Tang, Yaohua Zhang, Jiantian Leng, Jingjing Ma, Jingjing Ma, Quan Wang, Quan Wang, Xianzhong Feng   +11 more
doaj   +1 more source

Apparent Reversal of Allosteric Response in Mycobacterium tuberculosis MenD Reveals Links to Half‐of‐Sites Reactivity

ChemBioChem, Volume 26, Issue 7, April 1, 2025.
A combination of computational, biochemical and structural studies on allostery mutants of Mtb‐MenD, a menaquinone (vitamin K2) biosynthesis enzyme from a human pathogen, yields insight into the interrelationship between allosteric and half‐of‐sites reactivity mechanisms.
Ngoc Anh Thu Ho, Fiona M. Given, Tamsyn Stanborough, Michelle Klein, Timothy M. Allison, Esther M. M. Bulloch, Wanting Jiao, Jodie M. Johnston   +7 more
wiley   +1 more source

Pathway Editing Targets for Thiamine Biofortification in Rice Grains

Frontiers in Plant Science, 2018
Thiamine deficiency is common in populations consuming polished rice as a major source of carbohydrates. Thiamine is required to synthesize thiamine pyrophosphate (TPP), an essential cofactor of enzymes of central metabolism. Its biosynthesis pathway has
Anu P. Minhas, Rakesh Tuli, Sanjeev Puri
doaj   +1 more source

Expanding the Enzymatic Toolbox for Carboligation: Increasing the Diversity of the ‘Split’ Transketolase Sequence Space

ChemBioChem, Volume 26, Issue 6, March 15, 2025.
Several putative split transketolases were retrieved via (meta)genome mining, increasing our understanding of this type of prokaryotic TK, whose domains are located on two separate subunits. Ten split TKs were purified and tested for erythrulose production, with the best‐performing enzyme kinetically characterized.
Alessia Tonoli, Silvia Anselmi, John M. Ward, Helen C. Hailes, Jack W. E. Jeffries   +4 more
wiley   +1 more source

Metabolic Benefits of Six-month Thiamine Supplementation in Patients with and without Diabetes Mellitus Type 2

Clinical Medicine Insights: Endocrinology and Diabetes, 2014
Thiamine deficiency has been documented to be prevalent in patients with diabetes mellitus, and correction of thiamine deficiency in this population may provide beneficial effects in several cardiometabolic parameters, including prevention of impending ...
Omar Al-Attas, Nasser Al-Daghri, Majed Alokail, Sherif Abd-Alrahman, Benjamin Vinodson, Shaun Sabico   +5 more
doaj   +1 more source

Designing Enzymatic Reactivity with an Expanded Palette

ChemBioChem, EarlyView.
Innovation in biocatalysis is rapidly increasingly the diversity of catalytic reactivity that can be mediated by enzymes, addressing a key bottleneck for their widespread adoption in industrial chemical synthesis. A key approach to this is building enzymes with unnatural catalytic components that provide an expanded palette with new possibilities for ...
Reuben B. Leveson‐Gower
wiley   +1 more source

Potent Radical-Scavenging Activities of Thiamin and Thiamin Diphosphate

Journal of Clinical Biochemistry and Nutrition, 2007
Various radical-scavenging activities of thiamin and thiamin diphosphate (TDP) were found in some in vitro experiments. Thiamin and TDP caused considerable suppressive effects on superoxide generation in hypoxanthine and xanthine oxidase system which was measured by a sensitive chemiluminescence method using 2-methyl-6-[p-methylphenyl]-3,7 ...
Yasuji Okai, Eisuke F. Sato, Kiyoka Higashi-Okai, Masayasu Inoue, Ryusei Konaka   +4 more
openaire   +4 more sources

Diversity Of ThDP‐Dependent Enzymes Forming Chiral Tertiary Alcohols

ChemBioChem, Accepted Article.
Thiamine diphosphate (ThDP)‐dependent enzymes are well known biocatalysts for C−C bond‐forming reactions. While this enzyme class is mainly investigated for the formation of acyloins of secondary alcohols, recent studies have expanded its scope to utilize ketones as electrophiles in asymmetric carboligation reactions for the formation of tertiary ...
Daniela Bjarnesen, Lucrezia Lanza, Francesco Presini, Pier Paolo Giovannini, Michael Müller   +4 more
wiley   +1 more source

CONFIRMATION OF THE DEGRADATION PRODUCTS OF THIAMINE BY THIAMINE-DECOMPOSING THERMOSTABLE FACTOR AND THE MECHANISM OF THIAMINE DEGRADATION

THE JOURNAL OF VITAMINOLOGY, 1958
1. “Catechothiamine” and “Hydroquinothiamine”, thiamine degradation products by pyrocatechol and hydroquinone respectively, were isolated in a crystalline form. They were proved to be identical with “Rutinothiamine”. It was finally demostrated that they were all identical with thiamine disulfide of Zima by ultraviolet and infrared absorption spectra ...
Shiro Hosoda, Akiji Fujita, Eiichi Hasegawa   +2 more
openaire   +4 more sources

Biosynthesis of Ephedrine is Initiated by Pyridoxal Phosphate‐Dependent Formation of Cathinone

ChemBioChem, Accepted Article.
Ephedra alkaloids possess some of the most basic structures of alkaloids. Despite their importance for human use and their commercial relevance, the biosynthesis of ephedra alkaloids has remained enigmatic. The predominant biosynthetic pathway in the literature proposes a thiamine‐dependent caboligation followed by a transaminase, although no candidate
Karina Witte, Anne Behrens, Hannes M. Schwelm, Volker Auwärter, Michael Müller   +4 more
wiley   +1 more source