Results 241 to 250 of about 53,903 (284)
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Journal of Muscle Research and Cell Motility, 1989
Antibodies reacting with chicken gizzard caldesmon were used to determine the distribution of caldesmon on smooth muscle thin filaments. Antibodies developed against both the intact caldesmon molecule and a 40 kilodalton proteolytic fragment cause thin filaments to aggregate laterally.
W, Lehman, R, Craig, J, Lui, C, Moody
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Antibodies reacting with chicken gizzard caldesmon were used to determine the distribution of caldesmon on smooth muscle thin filaments. Antibodies developed against both the intact caldesmon molecule and a 40 kilodalton proteolytic fragment cause thin filaments to aggregate laterally.
W, Lehman, R, Craig, J, Lui, C, Moody
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Three-dimensional reconstruction of F-actin, thin filaments and decorated thin filaments
Journal of Molecular Biology, 1970Abstract The characteristic “arrowheads” appearance seen in electron micrographs of negatively-stained F-actin filaments when “decorated” with myosin or heavy meromyosin, has shown the structural polarity of actin, but it was not possible previously to deduce from such images the detailed way in which the individual myosin units were attached to the ...
P B, Moore, H E, Huxley, D J, DeRosier
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Analytical Biochemistry, 2010
We have developed a technique by which muscle thin filaments are reconstituted from the recombinant troponin components and the native thin filaments. By this technique, the reconstituted troponin complex is exchanged into the native thin filaments in the presence of 20% glycerol and 0.3M KCl at pH 6.2.
Fumiko, Matsumoto +3 more
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We have developed a technique by which muscle thin filaments are reconstituted from the recombinant troponin components and the native thin filaments. By this technique, the reconstituted troponin complex is exchanged into the native thin filaments in the presence of 20% glycerol and 0.3M KCl at pH 6.2.
Fumiko, Matsumoto +3 more
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Journal of Muscle Research and Cell Motility, 1990
Native and synthetic vertebrate smooth muscle thin filaments have been examined by electron microscopy in order to determine the arrangement of the regulatory protein caldesmon. In synthetic filaments of actin-caldesmon, long slender molecules were sometimes seen running along the thin filament, suggesting that caldesmon can associate with actin along ...
C, Moody, W, Lehman, R, Craig
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Native and synthetic vertebrate smooth muscle thin filaments have been examined by electron microscopy in order to determine the arrangement of the regulatory protein caldesmon. In synthetic filaments of actin-caldesmon, long slender molecules were sometimes seen running along the thin filament, suggesting that caldesmon can associate with actin along ...
C, Moody, W, Lehman, R, Craig
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Structure of myosin decorated actin filaments and natural thin filaments
Journal of Muscle Research and Cell Motility, 1985Negatively stained paracrystals of reconstituted thin filaments decorated with myosin subfragment 1 (S1), at high calcium concentrations (greater than or equal to 10(-5) M), exhibit pgg plane group symmetry with component filaments having 28 subunits in 13 turns of the actin genetic helix. Isolated S1 decorated F-actin filaments trapped in a stain film
J, Seymour, E J, O'Brien
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Structural analysis of muscle thin filament
Advances in Biophysics, 1991Thin sheets of Ac-Tm-Tn paracrystals were prepared in the presence of high concentration of Ca2+ ion and three-dimensional image analysis was performed. The optical diffraction pattern of an electron micrograph showed spots up to 1/1.6 nm-1 in the radial direction and up to 1/2.5 nm-1 in the axial direction, the best resolution ever obtained so far ...
A, Tomioka +6 more
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The position of tropomyosin in muscle thin filaments
Nature, 1980The contraction of vertebrate skeletal muscle is controlled by the action of Ca2+ on muscle thin filaments. At low Ca2+ concentrations (less than 10(-6)M) the regulatory proteins of the thin filament, tropomyosin and troponin, relax muscle by preventing the interaction of myosin and actin.
J, Seymour, E J, O'Brien
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On the Grain Boundary Grooving in Thin Filaments
Defect and Diffusion Forum, 2005We considered a polycrystalline cylindrical wire of the initial radius R0 composed of identical cylindrical grains of the length L0, strained uniaxially by an external stress P. At the temperatures at which some surface and grain boundary diffusion are allowed the thinning of the nanowire in the vicinity of grain boundaries occurs due to the phenomenon
Leonid Klinger, Eugen Rabkin
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The stoichiometry of the components of arthropod thin filaments
Biochimica et Biophysica Acta (BBA) - Protein Structure, 1976Limulus thin filaments confer calcium sensitivity on calcium-independent myosins and contain in addition to actin and tropomyosin, three troponin components. The molar ratio of actin:tropomyosin:troponin sub-unit T (TN-T): troponin sub-unit C (TN-C) is approximately 7:1:1:1, as in vertebrates, but twice the amount of the troponin sub-unit I (TN-I) may ...
W, Lehman, J M, Regenstein, A L, Ransom
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Nuclear Lamins: Thin Filaments with Major Functions
Trends in Cell Biology, 2018The nuclear lamina is a nuclear peripheral meshwork that is mainly composed of nuclear lamins, although a small fraction of lamins also localizes throughout the nucleoplasm. Lamins are classified as type V intermediate filament (IF) proteins. Mutations in lamin genes cause at least 15 distinct human diseases, collectively termed laminopathies ...
de Leeuw, Rebecca +2 more
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