Results 61 to 70 of about 19,795 (176)

The architecture of redox microdomains: Cascading gradients and peroxiredoxins’ redox-oligomeric coupling integrate redox signaling and antioxidant protection

Redox Biology
In the cytosol of human cells under low oxidative loads, hydrogen peroxide is confined to microdomains around its supply sites, due to its fast consumption by peroxiredoxins.
Matthew Griffith, Adérito Araújo, Rui Travasso, Armindo Salvador   +3 more
doaj   +1 more source

The Circadian Oscillator Affects Both Glutathione Homeostasis and Its Response to Salt Stress in Arabidopsis thaliana

Plant, Cell &Environment, EarlyView.
ABSTRACT Glutathione, including reduced glutathione (GSH) and oxidized glutathione (GSSG), is the major non‐protein thiol‐based redox buffer. There are diurnal rhythms in total glutathione level and in this work, we investigate whether these oscillations might be indicative of circadian regulation of total glutathione homeostasis.
Desiré Cano‐Yelo, Raquel López‐Martínez, Francisca Sevilla, Alex A. R. Webb, Ana Jiménez, María Carmen Martí   +5 more
wiley   +1 more source

The Role of Cysteine Residues in Redox Regulation and Protein Stability of Arabidopsis thaliana Starch Synthase 1. [PDF]

PLoS ONE, 2015
Starch biosynthesis in Arabidopsis thaliana is strictly regulated. In leaf extracts, starch synthase 1 (AtSS1) responds to the redox potential within a physiologically relevant range. This study presents data testing two main hypotheses: 1) that specific
Katsiaryna Skryhan, Jose A Cuesta-Seijo, Morten M Nielsen, Lucia Marri, Silas B Mellor, Mikkel A Glaring, Poul E Jensen, Monica M Palcic, Andreas Blennow   +8 more
doaj   +1 more source

1-Chloro-2,4-dinitrobenzene is an irreversible inhibitor of human thioredoxin reductase. Loss of thioredoxin disulfide reductase activity is accompanied by a large increase in NADPH oxidase activity.

The Journal of biological chemistry, 1995
Human thioredoxin reductase is a dimeric enzyme that catalyzes reduction of the disulfide in oxidized thioredoxin by a mechanism involving transfer of electrons from NADPH via FAD to a redox-active disulfide. 1-Chloro-2,4-dinitrobenzene (DNCB) is an alkylating agent used for depleting intracellular GSH and also showing distinct immunomodulatory ...
E S, Arnér, M, Björnstedt, A, Holmgren
openaire   +1 more source

The Thioredoxin System of Mammalian Cells and Its Modulators

Biomedicines, 2022
Oxidative stress involves the increased production and accumulation of free radicals, peroxides, and other metabolites that are collectively termed reactive oxygen species (ROS), which are produced as by-products of aerobic respiration.
Aseel Ali Hasan, Elena Kalinina, Victor Tatarskiy, Alexander Shtil   +3 more
doaj   +1 more source

How thioredoxin dissociates its mixed disulfide.

PLoS Computational Biology, 2009
The dissociation mechanism of the thioredoxin (Trx) mixed disulfide complexes is unknown and has been debated for more than twenty years. Specifically, opposing arguments for the activation of the nucleophilic cysteine as a thiolate during the ...
Goedele Roos, Nicolas Foloppe, Koen Van Laer, Lode Wyns, Lennart Nilsson, Paul Geerlings, Joris Messens   +6 more
doaj   +1 more source

From the Proteome to Therapeutics: A Multi‐Database Approach to Drug Discovery in Periodontitis—An Exploratory Pilot Study

Journal of Clinical Periodontology, Volume 53, Issue 7, Page 990-1004, July 2026.
ABSTRACT Introduction This explanatory pilot study presents a workflow to identify approved drugs, which could be repurposed for periodontitis therapy using salivary proteomics combined with drug‐target database screening. Methods Proteomic analyses of saliva using LC–MS/MS were conducted in two independent settings: a cohort (sub‐study I, N = 187) and
Taisir Bozo, Annette Murr, Birte Holtfreter, Sebastian‐Edgar Baumeister, Peter Meisel, Uwe Völker, Werner Weitschies, Henry Völzke, Philipp Kanzow, Elke Hammer, Manuela Gesell Salazar, Thomas Kocher   +11 more
wiley   +1 more source

Crystal Structure of the Apo-Form of NADPH-Dependent Thioredoxin Reductase from a Methane-Producing Archaeon

Antioxidants, 2018
The redox regulation of proteins via reversible dithiol/disulfide exchange reactions involves the thioredoxin system, which is composed of a reductant, a thioredoxin reductase (TR), and thioredoxin (Trx).
Rubén M. Buey, Ruth A. Schmitz, Bob B. Buchanan, Monica Balsera   +3 more
doaj   +1 more source

Cars2‐Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2

Advanced Science, Volume 13, Issue 31, 4 June 2026.
We demonstrate that Cars2, a cysteine catabolic enzyme in mouse iBAT, is critical for cold tolerance and brown adipocyte differentiation. Through its CPERS activity, Cars2 produces CysSSH/H2S to induce EBF2 persulfidation, promoting its interaction with PPARγ and BRG1 to enhance thermogenic gene expression.
Xin Peng, Hongyong Song, Yin Long, Kaimin Wu, Yinkun Fu, Wenkai Zhou, Wenyan Li, Zhe Shi, Xu‐Yun Zhao   +8 more
wiley   +1 more source

Santamarine Synergizes With Cisplatin via ROS/JNK Axis to Selectively Induce Apoptosis and DNA Damage in Oral Cancer Cells In Vitro

Drug Development Research, Volume 87, Issue 4, June 2026.
ABSTRACT Santamarine (SAMA), a Michelia compressa‐derived sesquiterpene lactone, exhibits ROS‑mediated anticancer activity; however, its potential mechanisms and synergy with cisplatin in oral cancer have not been explored. This study investigated SAMA and cisplatin's combined effects and underlying ROS/MAPK mechanisms of action on oral cancer cells ...
Hsin‐I. Lu, Kuan‐Liang Chen, Chung‐Yi Chen, Ching‐Yu Yen, Ammad Ahmad Farooqi, Tsu‐Ming Chien, Jiiang‐Huei Jeng, Bing‐Hung Chen, Hsueh‐Wei Chang   +8 more
wiley   +1 more source