Results 101 to 110 of about 884,746 (312)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

AccuVIR: an ACCUrate VIRal genome assembly tool for third-generation sequencing data

Bioinform., 2022
Motivation RNA viruses tend to mutate constantly. While many of the variants are neutral, some can lead to higher transmissibility or virulence. Accurate assembly of complete viral genomes enables the identification of underlying variants, which are ...
Runzhou Yu, Dehan Cai, Yanni Sun
semanticscholar   +1 more source

Third-generation Sequencing Reveals Extensive Polycistronism and Transcriptional Overlapping in a Baculovirus [PDF]

, 2018
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is an insect-pathogen baculovirus. In this study, we applied the Oxford Nanopore Technologies platform for the analysis of the polyadenylated fraction of the viral transcriptome using both
Balázs, Zsolt, Boldogkői, Zsolt, Csabai, Zsolt, Moldován, Norbert, Szűcs, Attila, Tombácz, Dóra   +5 more
core   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Micropathogen community identification in ticks (Acari: Ixodidae) using third-generation sequencing

International Journal for Parasitology: Parasites and Wildlife, 2021
Ticks are important vectors that facilitate the transmission of a broad range of micropathogens to vertebrates, including humans. Because of their role in disease transmission, it has become increasingly important to identify and characterize the ...
Jin Luo, Qiaoyun Ren, Wenge Liu, Xiangrui Li, Hong Yin, Mingxin Song, Bo Zhao, Guiquan Guan, Jianxun Luo, Guangyuan Liu   +9 more
doaj   +1 more source

Applications of next-generation sequencing technologies and computational tools in molecular evolution and aquatic animals conservation studies : a short review [PDF]

, 2019
Aquatic ecosystems that form major biodiversity hotspots are critically threatened due to environmental and anthropogenic stressors. We believe that, in this genomic era, computational methods can be applied to promote aquatic biodiversity conservation ...
Afiqah-Aleng, Nor, Danish-Daniel, Muhd, Mohd Nor, Siti Azizah, Razali, Siti Aisyah, Sorgeloos, Patrick, Sung, Yeong Yik, Tan, Min Pau, Van de Peer, Yves, Wong, Li Lian   +8 more
core   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Implementing Whole Genome Sequencing (WGS) in Clinical Practice: Advantages, Challenges, and Future Perspectives

Cells
The integration of whole genome sequencing (WGS) into all aspects of modern medicine represents the next step in the evolution of healthcare. Using this technology, scientists and physicians can observe the entire human genome comprehensively, generating
Petar Brlek, Luka Bulić, Matea Bračić, Petar Projić, Vedrana Škaro, Nidhi Shah, Parth Shah, Dragan Primorac   +7 more
doaj   +1 more source

DNA/RNA transverse current sequencing: Intrinsic structural noise from neighboring bases

Frontiers in Genetics, 2015
Nanopore DNA sequencing via transverse current has emerged as a promising candidate for third-generation sequencing technology. It produces long read lengths which could alleviate problems with assembly errors inherent in current technologies.
Jose eAlvarez, Dmitry eSkachkov, Steven eMassey, Alan eKalitsov, Julian eVelev   +4 more
doaj   +1 more source

Gene sequencing and result analysis of balanced translocation carriers by third-generation gene sequencing technology

Scientific Reports, 2023
Because the total gene copy number remains constant and all genes are normally expressed, carriers of balanced chromosomal translocations usually have a normal phenotype but are able to produce many different types of gametes during meiosis, and ...
Xiaoqi Zeng, Dandan Lin, Danhong Liang, Jingwen Huang, Jinsong Yi, Dianliang Lin, Zhengmian Zhang   +6 more
doaj   +1 more source