Results 111 to 120 of about 6,398,546 (257)

Diagnosis of external ventricular drainage related infections with real-time 16S PCR and third-generation 16S sequencing

Investigate the performance of real-time 16S PCR and third-generation 16S sequencing in the diagnosis of external ventricular drain related infections (EVDRI).
Johan Widén (560740), Anna Fagerström (3594854), Britt-Marie Eriksson (470276), Elisabeth Ronne-Engström (2831642), Paula Mölling (471622), Per Enblad (839343), Martin Sundqvist (367060), Gabriel Westman (470272), Jakob Morén (17889172)   +8 more
core   +2 more sources

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

The Role of Next Generation Sequencing in Genetic Counseling

, 2016
Genetic counseling is defined as a process that gives information about the risk of developing or transmitting a genetic condition to the next generation and management or treatment options of the genetic ailment.
Asude Durmaz, Durmaz A., Burak Durmaz, Durmaz B.   +3 more
core   +1 more source

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

The Third-Generation Sequencing Challenge: Novel Insights for the Omic Sciences. [PDF]

Biomolecules
The understanding of the human genome has been greatly improved by the advent of next-generation sequencing technologies (NGS). Despite the undeniable advantages responsible for their widespread diffusion, these methods have some constraints, mainly ...
Scarano C, Veneruso I, De Simone RR, Di Bonito G, Secondino A, D'Argenio V.   +5 more
europepmc   +2 more sources

The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection

Molecular Oncology, EarlyView.
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński, Krzysztof Pastuszak, Anna Samelak‐Czajka, Zuzanna Cemka, Paulina Jackowiak, Tomasz Stefaniak, Sylwia Łapińska‐Szumczyk, Jacek Jassem, Anna J. Żaczek, Matthew T. Rondina, Myron G. Best, Sjors G. J. G. In 't Veld, Anna Supernat   +12 more
wiley   +1 more source

Symposium NGS 2016 - ‘Next generation sequencing’ technology in routine analysis in Belgian healthcare system (EN)

, 2016
‘Next generation sequencing’ technology in routine analysis in Belgian healthcare system.
M. Van den Bulcke
core  

SMART-RNA-Metavirome: a practical RNA metavirome platform compatible with high-throughput sequencing of both short and long reads

Infectious Diseases of Poverty
Background The RNA virosphere's extensive diversity and its role in emerging infectious diseases underscore the importance of non-targeted sequencing for identifying unknown or rare pathogens, including co-infections.
Xiaohua Liu, Ziyao Li, Xiang Guo, Liu Ge, Minling Hu, Qing He, Xiaoqing Zhang, Ziqing Feng, Yuji Wang, Lingzhai Zhao, Shu Zeng, Wenwen Ren, Haiyang Chen, Chunmei Wang, Rangke Wu, Wei Zhao, Fuchun Zhang, Xiao-Guang Chen, Xiaohong Zhou   +18 more
doaj   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Analysis of gene expresion via next generation sequencing techniques [PDF]

, 2014
1 Abstract The main task of this work is create review today's available methods for gene expression analysis, introduce advantages and disadvantages of this metods and compare them.
Bláhová, Monika
core