Results 61 to 70 of about 884,746 (312)

DNA Preparation In Vitis Vinifera L. for Third Generation Sequencing [PDF]

, 2021
Abstract Background: There have been several attempts to sequence the genome of Vitis vinifera L. (grapevine), utilizing low-resolution second-generation platforms. Nevertheless, the characterization of the grapevine genetic resources and its adaptation to vulnerable conditions could be better addressed through extensive and high-resolution ...
Bibi C. Androniki, Kollias Anastasios, Astrinaki Maria, Vassou Despoina, Kafetzopoulos Dimitris, Kalantidis Kriton, Moschou N. Panagiotis   +6 more
openaire   +3 more sources

Advances in Integrating Genomics and Bioinformatics in the Plant Breeding Pipeline

Agriculture, 2018
With the global human population growing rapidly, agricultural production must increase to meet crop demand. Improving crops through breeding is a sustainable approach to increase yield and yield stability without intensifying the use of fertilisers and ...
Haifei Hu, Armin Scheben, David Edwards
doaj   +1 more source

From Samples to Germline and Somatic Sequence Variation: A Focus on Next-Generation Sequencing in Melanoma Research

Life, 2022
Next-generation sequencing (NGS) applications have flourished in the last decade, permitting the identification of cancer driver genes and profoundly expanding the possibilities of genomic studies of cancer, including melanoma. Here we aimed to present a
Adrián Muñoz-Barrera, Luis A. Rubio-Rodríguez, Ana Díaz-de Usera, David Jáspez, José M. Lorenzo-Salazar, Rafaela González-Montelongo, Víctor García-Olivares, Carlos Flores   +7 more
doaj   +1 more source

High-throughput method for the hybridisation-based targeted enrichment of long genomic fragments for PacBio third-generation sequencing

NAR Genomics and Bioinformatics, 2022
Hybridisation-based targeted enrichment is a widely used and well-established technique in high-throughput second-generation short-read sequencing. Despite the high potential to genetically resolve highly repetitive and variable genomic sequences by, for
T. Steiert, J. Fuss, S. Juzėnas, M. Wittig, M. Hoeppner, Melanie Vollstedt, G. Varkalaite, H. ElAbd, C. Brockmann, S. Görg, Christoph Gassner, M. Forster, A. Franke   +12 more
semanticscholar   +1 more source

Comparison of TCGA and GENIE genomic datasets for the detection of clinically actionable alterations in breast cancer. [PDF]

, 2019
Whole exome sequencing (WES), targeted gene panel sequencing and single nucleotide polymorphism (SNP) arrays are increasingly used for the identification of actionable alterations that are critical to cancer care.
Carpten, John D, Kaur, Pushpinder, Lang, Julie E, Porras, Tania B, Ring, Alexander   +4 more
core   +3 more sources

A practical, bioinformatic workflow system for large data sets generated by next-generation sequencing [PDF]

, 2010
Transcriptomics (at the level of single cells, tissues and/or whole organisms) underpins many fields of biomedical science, from understanding the basic cellular function in model organisms, to the elucidation of the biological events that govern the ...
Aaron R. Jex, Altschul, Anja Joachim, Ashburner, Bentley, Bethony, Björnberg, Blaxter, Boag, Bronwyn E. Campbell, Caffrey, Campbell, Cantacessi, Cantacessi, Cantacessi, Cantacessi, Chan, Chang, Cinzia Cantacessi, Clifton, Conesa, Cottee, Cottee, Datu, DeRisi, Doyle, Flicek, Freigofas, Gasser, Golden, Greene, Gupta, Hawdon, Hopkins, Hotez, Hu, Huang, Hunter, Iseli, Jackson, Joachim, Joachim, Keil, Krasky, Letunic, Li, Li, Li, Lipinski, Makedonka Mitreva, Margulies, Matthew J. Nolan, McKay, Metzker, Miller, Miller, Mizuarai, Moreno, Morozova, Moser, Mufson, Mulvenna, Nagaraj, Nagaraj, Neil D. Young, Nikolaou, Nisbet, Olson, Parkinson, Paul W. Sternberg, Pong, Portman, Ranganathan, Ren, Robertson, Robin B. Gasser, Robinson, Ross S. Hall, Sahar Abubucker, Sanger, Sanger, Santos, Shoba Ranganathan, Soderlund, Stathopoulos, Stockdale, Tanaka, Vibranovski, Wang, Williamson, Wilson, Wu, Young, Young, Zhan, Zhong   +95 more
core   +6 more sources

An updated database of virus circular RNAs provides new insights into the biogenesis mechanism of the molecule

Emerging Microbes and Infections, 2023
Virus circular RNAs (circRNA) have been reported to be extensively expressed and play important roles in viral infections. Previously we build the first database of virus circRNAs named VirusCircBase which has been widely used in the field.
Ping Fu, Zena Cai, Zhiyuan Zhang, Xiangxian Meng, Yousong Peng   +4 more
doaj   +1 more source

Third-Generation Sequencing as a New Comprehensive Technology for Identifying Rare α- and β-Globin Gene Variants in Thalassemia Alleles in the Chinese Population.

Archives of Pathology & Laboratory Medicine, 2022
CONTEXT.— Identification of rare thalassemia variants requires a combination of multiple diagnostic technologies. OBJECTIVE.— To investigate a new approach of comprehensive analysis of thalassemia alleles based on third-generation sequencing (TGS) for ...
J. Zhuang, Chunnuan Chen, Wanyu Fu, Yuanbai Wang, Qianmei Zhuang, Yulin Lu, T. Xie, Ruofan Xu, Shuhong Zeng, Yuying Jiang, Yingjun Xie, Gaoxiong Wang   +11 more
semanticscholar   +1 more source

NGSpeciesID: DNA barcode and amplicon consensus generation from long‐read sequencing data

Ecology and Evolution, 2021
Third‐generation sequencing technologies, such as Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), have gained popularity over the last years. These platforms can generate millions of long‐read sequences. This is not only advantageous
Kristoffer Sahlin, Marisa C. W. Lim, Stefan Prost   +2 more
doaj   +1 more source

Analyzing Modern Biomolecules: The Revolution of Nucleic-Acid Sequencing – Review

Biomolecules, 2021
Recent developments have revolutionized the study of biomolecules. Among them are molecular markers, amplification and sequencing of nucleic acids. The latter is classified into three generations. The first allows to sequence small DNA fragments.
Gabriel Dorado, Sergio Gálvez, Teresa E. Rosales, Víctor F. Vásquez, Pilar Hernández   +4 more
doaj   +1 more source