Results 71 to 80 of about 884,760 (321)

Analyzing Modern Biomolecules: The Revolution of Nucleic-Acid Sequencing – Review

Biomolecules, 2021
Recent developments have revolutionized the study of biomolecules. Among them are molecular markers, amplification and sequencing of nucleic acids. The latter is classified into three generations. The first allows to sequence small DNA fragments.
Gabriel Dorado, Sergio Gálvez, Teresa E. Rosales, Víctor F. Vásquez, Pilar Hernández   +4 more
doaj   +1 more source

A hybrid correcting method considering heterozygous variations by a comprehensive probabilistic model

BMC Genomics, 2020
Background The emergence of the third generation sequencing technology, featuring longer read lengths, has demonstrated great advancement compared to the next generation sequencing technology and greatly promoted the biological research.
Jiaqi Liu, Jiayin Wang, Xiao Xiao, Xin Lai, Daocheng Dai, Xuanping Zhang, Xiaoyan Zhu, Zhongmeng Zhao, Juan Wang, Zhimin Li   +9 more
doaj   +1 more source

The red thread between methylation and mutation in bacterial antibiotic resistance: How third-generation sequencing can help to unravel this relationship

Frontiers in Microbiology, 2022
DNA methylation is an important mechanism involved in bacteria limiting foreign DNA acquisition, maintenance of mobile genetic elements, DNA mismatch repair, and gene expression.
Stella Papaleo, Alessandro Alvaro, Riccardo Nodari, S. Panelli, I. Bitar, F. Comandatore   +5 more
semanticscholar   +1 more source

A case of recurrent epilepsy-associated rosette-forming glioneuronal tumor with anaplastic transformation in the absence of therapy. [PDF]

, 2019
Rosette-forming glioneuronal tumor (RGNT) most commonly occurs adjacent to the fourth ventricle and therefore rarely presents with epilepsy. Recent reports describe RGNT occurrence in other anatomical locations with considerable morphologic and genetic ...
Corless, Christopher L, Ferris, Sean P, Grafe, Marjorie, Gultekin, Sakir H, Halfpenny, Aaron, Lawce, Helen, Lucas, Lora, Moore, Stephen, Nazemi, Kellie, Olson, Susan, Perry, Arie, Selden, Nathan, Solomon, David A, Woltjer, Randy, Wood, Matthew D   +14 more
core   +1 more source

Evolutionary Insight into the Trypanosomatidae Using Alignment-Free Phylogenomics of the Kinetoplast

Pathogens, 2019
Advancements in next-generation sequencing techniques have led to a substantial increase in the genomic information available for analyses in evolutionary biology.
Alexa Kaufer, Damien Stark, John Ellis
doaj   +1 more source

Long walk to genomics: History and current approaches to genome sequencing and assembly

Computational and Structural Biotechnology Journal, 2020
Genomes represent the starting point of genetic studies. Since the discovery of DNA structure, scientists have devoted great efforts to determine their sequence in an exact way.
Alice Maria Giani, Guido Roberto Gallo, Luca Gianfranceschi, Giulio Formenti   +3 more
doaj   +1 more source

Improvement of large copy number variant detection by whole genome nanopore sequencing

Journal of Advanced Research, 2023
Introduction: Whole-genome sequencing using nanopore technologies can uncover structural variants, which are DNA rearrangements larger than 50 base pairs.
Javier Cuenca-Guardiola, Belén de la Morena-Barrio, Juan L. García, Alba Sanchis-Juan, Javier Corral, Jesualdo T. Fernández-Breis   +5 more
doaj   +1 more source

DNA Preparation In Vitis Vinifera L. for Third Generation Sequencing [PDF]

, 2021
Abstract Background: There have been several attempts to sequence the genome of Vitis vinifera L. (grapevine), utilizing low-resolution second-generation platforms. Nevertheless, the characterization of the grapevine genetic resources and its adaptation to vulnerable conditions could be better addressed through extensive and high-resolution ...
Bibi C. Androniki, Kollias Anastasios, Astrinaki Maria, Vassou Despoina, Kafetzopoulos Dimitris, Kalantidis Kriton, Moschou N. Panagiotis   +6 more
openaire   +3 more sources

Uncovering 1,058 novel human enteric DNA viruses through deep long-read third-generation sequencing and their clinical impact.

Gastroenterology, 2022
BACKGROUND & AIMS Lack of viral reference genomes poses a challenge to virome study. We investigated human gut virome and its clinical implication by ultra-deep metagenomic sequencing.
Liuyang Zhao, Yu Shi, Harry Cheuk-Hay Lau, Weixin Liu, Guangwen Luo, Guoping Wang, Chang-an Liu, Yasi Pan, Qiming Zhou, Yanqiang Ding, Joseph Jao-Yiu Sung, Jun Yu   +11 more
semanticscholar   +1 more source

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources