Results 81 to 90 of about 574,833 (290)

Micropathogen community identification in ticks (Acari: Ixodidae) using third-generation sequencing

International Journal for Parasitology: Parasites and Wildlife, 2021
Ticks are important vectors that facilitate the transmission of a broad range of micropathogens to vertebrates, including humans. Because of their role in disease transmission, it has become increasingly important to identify and characterize the ...
Jin Luo, Qiaoyun Ren, Wenge Liu, Xiangrui Li, Hong Yin, Mingxin Song, Bo Zhao, Guiquan Guan, Jianxun Luo, Guangyuan Liu   +9 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

A Sequence-Based Novel Approach for Quality Evaluation of Third-Generation Sequencing Reads [PDF]

Genes, 2019
The advent of third-generation sequencing (TGS) technologies, such as the Pacific Biosciences (PacBio) and Oxford Nanopore machines, provides new possibilities for contig assembly, scaffolding, and high-performance computing in bioinformatics due to its long reads.
Zhang, Wenjing, Huang, Neng, Zheng, Jiantao, Liao, Xingyu, Wang, Jianxin, Li, Hong-Dong   +5 more
openaire   +2 more sources

Gene sequencing and result analysis of balanced translocation carriers by third-generation gene sequencing technology

Scientific Reports, 2023
Because the total gene copy number remains constant and all genes are normally expressed, carriers of balanced chromosomal translocations usually have a normal phenotype but are able to produce many different types of gametes during meiosis, and ...
Xiaoqi Zeng, Dandan Lin, Danhong Liang, Jingwen Huang, Jinsong Yi, Dianliang Lin, Zhengmian Zhang   +6 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Implementing Whole Genome Sequencing (WGS) in Clinical Practice: Advantages, Challenges, and Future Perspectives

Cells
The integration of whole genome sequencing (WGS) into all aspects of modern medicine represents the next step in the evolution of healthcare. Using this technology, scientists and physicians can observe the entire human genome comprehensively, generating
Petar Brlek, Luka Bulić, Matea Bračić, Petar Projić, Vedrana Škaro, Nidhi Shah, Parth Shah, Dragan Primorac   +7 more
doaj   +1 more source

DNA/RNA transverse current sequencing: Intrinsic structural noise from neighboring bases

Frontiers in Genetics, 2015
Nanopore DNA sequencing via transverse current has emerged as a promising candidate for third-generation sequencing technology. It produces long read lengths which could alleviate problems with assembly errors inherent in current technologies.
Jose eAlvarez, Dmitry eSkachkov, Steven eMassey, Alan eKalitsov, Julian eVelev   +4 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Third-Generation Cytogenetic Analysis: Diagnostic Application of Long-Read Sequencing.

The Journal of molecular diagnostics : JMD, 2022
Copy number variants (CNVs) play important roles in the pathogenesis of several genetic syndromes. Traditional and molecular karyotyping are considered the first-tier diagnostic tests to detect macroscopic and cryptic deletions/duplications. However, their time-consuming and laborious experimental protocols protract diagnostic times from 3 to 15 days ...
Magini, Pamela, Mingrino, Alessandra, Gega, Barbara, Mattei, Gianluca, Semeraro, Roberto, Bolognini, Davide, Mongelli, Patrizia, Desiderio, Laura, Pittalis, Maria Carla, Pippucci, Tommaso, Magi, Alberto   +10 more
openaire   +2 more sources