Results 71 to 80 of about 828,765 (377)

High density culturing of porcine hepatocytes immobilized on nonwoven polyurethane-based biomatrices [PDF]

, 2001
Objective: Hepatocytes are increasingly used as functional units in bioartificial liver devices. The objective of the present study was to investigate the feasibility of culturing porcine hepatocytes in high density on a novel polyurethane-based nonwoven
Castell, M. J., Doser, M., Gomez-Lechon, M. J., Koebe, H. G., Pahernik, S. A., Planck, H., Thasler, Wolfgang Erwin   +6 more
core   +1 more source

Developing Three Dimensional Culture System for Notochordal Cells

Global Spine Journal, 2012
Introduction The central compartment of the intervertebral disk (IVD) is nucleus pulposus (NP) which contains two cell types, large notochordal cells (NCCs) and small cartilage-like cells. Loss of NCCs is related to disk degeneration as these cells are required for the maintenance of NP.
Yeung, CW, Au, YK, Gao, Y, Chan, D, Cheah, KSE, Chan, BP   +5 more
openaire   +2 more sources

PuraMatrix hydrogel enhances the expression of motor neuron progenitor marker and improves adhesion and proliferation of motor neuron-like cells [PDF]

Iranian Journal of Basic Medical Sciences, 2020
Objective(s): Cell therapy has provided clinical applications to the treatment of motor neuron diseases. The current obstacle in stem cell therapy is to direct differentiation of stem cells into neurons in the neurodegenerative disorders.
Marzieh Darvishi, Hatef Ghasemi Hamidabadi, Sajad Sahab Negah, Ardeshir Moayeri, Taki Tiraihi, Javad Mirnajafi-Zadeh, Ali Jahanbazi Jahan-Abad, Amir Shojaei   +7 more
doaj   +1 more source

The Role of Patient-Derived Organoids in Triple-Negative Breast Cancer Drug Screening

Biomedicines, 2023
Triple-negative breast cancer (TNBC) is one of the most aggressive breast cancer subtypes, with a grave prognosis and few effective treatment options.
Iason Psilopatis, Amalia Mantzari, Kleio Vrettou, Stamatios Theocharis   +3 more
doaj   +1 more source

Three-dimensional tissue scaffolds from interbonded poly(e-caprolactone) fibrous matrices with controlled porosity

, 2011
In this article, we report on the preparation and cell culture performance of a novel fibrous matrix that has an interbonded fiber architecture, excellent pore interconnectivity, and controlled pore size and porosity.
Kirkland, Mark, Lin, Tong, Sutti, Alessandra, Tang, Yanwei, Wang, Hongxia, Wang, Xungai, Wong, Cynthia   +6 more
core   +1 more source

Three-dimensional cell culture models for investigating human viruses [PDF]

Virologica Sinica, 2016
Three-dimensional (3D) culture models are physiologically relevant, as they provide reproducible results, experimental flexibility and can be adapted for high-throughput experiments. Moreover, these models bridge the gap between traditional two-dimensional (2D) monolayer cultures and animal models.
He, Bing, Chen, Guomin, Zeng, Yi
openaire   +2 more sources

Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age

Infection and Immunity, 2018
Tissues and organs provide the structural and biochemical landscapes upon which microbial pathogens and commensals function to regulate health and disease. While flat two-dimensional (2-D) monolayers composed of a single cell type have provided important
Jennifer Barrila, A. Crabbé, Jiseon Yang, Karla Franco, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Sandhya Gangaraju, C. M. Ott, Carolyn B. Coyne, M. Bissell, C. Nickerson   +11 more
semanticscholar   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Effects of TGF-β1 on the migration of oral cancer-associated fibroblasts in two and three dimensional co-culture models

口腔疾病防治, 2020
Objective To observe the effect of transforming growth factor-β1 (TGF-β1) on the migration of oral carcinoma associated fibroblasts (CAFs) with two-dimensional culture model and three-dimensional model.Methods Under two-dimensional culture conditions ...
YANG Jin, WU Feifei, GAO Qinghong, LI Xiaoyu, MANABU Kato, CHENG Ran, ZHOU Hongmei   +6 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source