Results 91 to 100 of about 18,631,052 (336)

SPHINX31 acts as a SRPK1 inhibitor targeting the ATR/DNA‐PKcs/CHK1 replicative checkpoint to inhibit cell growth in non‐small cell lung cancer

Molecular Oncology, EarlyView.
The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim, Aurelie Genoux, Nadiia Zubchuk, Haoyang Zheng, Perla Salameh, Theo Ziegelmeyer, Fabien Dalonneau, Margot Rouchette, Christiane Oddou, Simona Miron, Helene Polveche, Adiilah Mamode Cassim, Carmen Garrido, Eleni Nikolakaki, Didier Auboeuf, Tao Jia, Sophie Zinn‐Justin, Beatrice Eymin   +17 more
wiley   +1 more source

Reprogramming tumor cells with LCOR mRNA nanotherapy to enhance immunotherapy

Molecular Oncology, EarlyView.
LCOR (Ligand‐dependent corepressor) mRNA encapsulated in polymeric nanoparticles restores LCOR activity in tumor cells, constraining aggressive tumor cell states and increasing tumor immunogenicity. This reprogramming makes tumor cells more vulnerable to T‐cell attack and enhances their response to immune‐checkpoint immunotherapy.
Gabriel Serra‐Mir, Elena Haro‐Martínez, Sandra Blasco‐Benito, Chiara Cannatà, José Ángel Palomeque, Anna López‐Plana, Maria Sanz‐Flores, Marc Sabartés, Cristina Fornaguera, Toni Celià‐Terrassa   +9 more
wiley   +1 more source

IN VITRO INVESTIGATION OF THE TRANSPLANTATION PROSPECTS OF MULTICELLULAR SPHEROID MICROAGGREGATES OF DONOR RETINAL PIGMENT EPITHELIUM

Вестник трансплантологии и искусственных органов, 2015
Aim. To study in experiment the criteria for transplantability of multicellular spheroid microaggregates of retinal pigment epithelium (RPE), prepared by the method of 3D cell culture. Materials and Methods. 11 donor eyes (6 of adrenaline index «A», 5 of
S. A. Borzenok, I. A. Popov, I. N. Saburina, P. M. Arbukhanova   +3 more
doaj   +1 more source

Three-dimensional cell culture: from evolution to revolution [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2018
Recent advances in the isolation of tissue-resident adult stem cells and the identification of inductive factors that efficiently direct differentiation of human pluripotent stem cells along specific lineages have facilitated the development of high-fidelity modelling of several tissues in vitro
Sharmin Alhaque, Michael Themis, Hassan Rashidi   +2 more
openaire   +4 more sources

Extreme-Point-based Heuristics for the Three-Dimensional Bin Packing problem [PDF]

, 2008
One of the main issues in addressing three-dimensional packing problems is finding an efficient and accurate definition of the points at which to place the items inside the bins, because the performance of exact and heuristic solution methods is actually
CRAINIC T. G, Crainic T.G, Perboli, Guido, Tadei, Roberto   +3 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Chapter 5 Three-Dimensional culture for Expansion and Differentiation of Embryonic Stem Cells

, 2011
Chapter 5 Three-Dimensional culture for Expansion and Differentiation of Embryonic Stem ...
待补充, 孙广炜, 马小军, 徐小溪, 张英, 李楠   +5 more
core  

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

De novo reconstruction of a functional in vivo-like equine endometrium using collagen-based tissue engineering

Scientific Reports
To better understand molecular aspects of equine endometrial function, there is a need for advanced in vitro culture systems that more closely imitate the intricate 3-dimensional (3D) in vivo endometrial structure than current techniques.
Sawita Santiviparat, Theerawat Swangchan-Uthai, Tom A. E. Stout, Supranee Buranapraditkun, Piyathip Setthawong, Teeanutree Taephatthanasagon, Watchareewan Rodprasert, Chenphop Sawangmake, Theerawat Tharasanit   +8 more
doaj   +1 more source

Establishment of a Porcine Small Intestinal Organoid Model for Porcine Rotavirus Infection

Animals
Porcine rotavirus (PoRV), a major enteric pathogen, causes severe diarrhea, dehydration, high morbidity, and mortality, leading to significant economic losses in the porcine industry.
Huangsiwu Wei, Wenjie Wu, Maokang Liu, Mengchen Li, Juan Zhang, Shunzhou Deng   +5 more
doaj   +1 more source