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Three-Dimensional Cell Culture: A Breakthrough in Vivo [PDF]
International Journal of Molecular Sciences, 2015 Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their
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Biochemical and Biophysical Research Communications - BBRC, 2020
Three-dimensional (3D) culture reflects tumor biology complexities compared with two-dimensional (2D) culture. Thus, 3D culture has attracted attention in cell biology studies including drug sensitivity tests.
M. Muguruma +9 more
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Three-dimensional (3D) culture reflects tumor biology complexities compared with two-dimensional (2D) culture. Thus, 3D culture has attracted attention in cell biology studies including drug sensitivity tests.
M. Muguruma +9 more
semanticscholar +1 more source
Gene, 2019
In recent years, in vitro three-dimensional (3D) cell culture systems that better mimic in vivo physiology and tissue microenvironment have become important.
Pelin Sağlam-Metiner +2 more
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In recent years, in vitro three-dimensional (3D) cell culture systems that better mimic in vivo physiology and tissue microenvironment have become important.
Pelin Sağlam-Metiner +2 more
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2000
Abstract Three-dimensional (30) cell cultures have been widely used in biomedical research since the early decades of this century. Holtfreter (1) and later Moscona (2, 3) pioneered the field by their research on morphogenesis using spherical reaggregated cultures of embryonic or malignant cells.
L A Kunz-Schughart, W Mueller-Kliesert
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Abstract Three-dimensional (30) cell cultures have been widely used in biomedical research since the early decades of this century. Holtfreter (1) and later Moscona (2, 3) pioneered the field by their research on morphogenesis using spherical reaggregated cultures of embryonic or malignant cells.
L A Kunz-Schughart, W Mueller-Kliesert
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Three-dimensional culture and bioreactors for cellular therapies
Cytotherapy, 2011A bioreactor is defined as a specifically designed vessel to facilitate the growth of organisms and cells through application of physical and/or electrical stimulus. When cells with therapeutic potential were first discovered, they were initially cultured and expanded in two-dimensional (2-D) culture vessels such as plates or T-flasks.
M W, Naing, D J, Williams
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Analysis of Cell Proliferation by Three-Dimensional Culture
2022Three-dimensional (3D) cell culture technology is a powerful tool in cancer research and drug development. It retains several critical components of the in vivo environment, including cell-cell and cell-extracellular matrix (ECM) interactions in a 3D fashion, gradients of oxygen, nutrients and metabolic waste, and it is thus more physiologically ...
Xiaoyun, Tang, David N, Brindley
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Three-dimensional microwell arrays for cell culture
Lab Chip, 2011We propose the concept of three-dimensional (3D) microwell arrays for cell culture applications and highlight the importance of oxygen diffusion through pores in all three dimensions to enhance cell viability.
Christina L, Randall +4 more
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Micropatterns of Matrigel for three-dimensional epithelial cultures
Biomaterials, 2007Three-dimensional (3D) epithelial culture models are widely used to promote a physiologically relevant microenvironment for the study of normal and aberrant epithelial organization. Despite the increased use of these models, their potential as a cell-based screening tool for therapeutics has been hindered by the lack of existing platforms for large ...
Temitope R, Sodunke +5 more
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The growth of axons in three-dimensional astrocyte cultures
Developmental Biology, 1989The environment of the adult central nervous system (CNS) does not support axon regeneration. We have been unable to replicate this behaviour using monolayer cultures of glia, so we have developed a technique for three dimensional culture of glial cells.
J W, Fawcett +3 more
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Three‐dimensional Cell Cultures Mimic Tissuesa
Annals of the New York Academy of Sciences, 1992Three-dimensional cell culture using gels of type I collagen is a flexible method for studying cell behavior in a tissuelike environment. With only small changes in the basic protocol, we were able to encapsulate neutrophils, hepatocytes, and PC12 cells.
W M, Saltzman +3 more
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