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Three-dimensional cell culturing by magnetic levitation
Nature Protocols, 2013Recently, biomedical research has moved toward cell culture in three dimensions to better recapitulate native cellular environments. This protocol describes one method for 3D culture, the magnetic levitation method (MLM), in which cells bind with a magnetic nanoparticle assembly overnight to render them magnetic. When resuspended in medium, an external
William L, Haisler +5 more
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Self-organizing optic-cup morphogenesis in three-dimensional culture
Nature, 2011M. Eiraku +8 more
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The rise of three-dimensional human brain cultures
Nature, 2018Pluripotent stem cells show a remarkable ability to self-organize and differentiate in vitro in three-dimensional aggregates, known as organoids or organ spheroids, and to recapitulate aspects of human brain development and function. Region-specific 3D brain cultures can be derived from any individual and assembled to model complex cell-cell ...
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Modeling Osteoarthritis Using Three-Dimensional Culture
, 2020Alexandra Fiederlein, Jodi F. Evans
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Methods: Using Three-Dimensional Culture (Spheroids) as an In Vitro Model of Tumour Hypoxia.
Advances in Experimental Medicine and Biology, 2016R. Leek +3 more
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