Results 91 to 100 of about 47,729 (258)

Label‐Free and Immobilization‐Free Protein‐Binding Assays by Ultraviolet Transient Absorption Microscopy

Advanced Science, EarlyView.
A femtosecond UV pump–probe microscope resolves binding‐induced changes in protein excited‐state dynamics and converts these intrinsic contrasts into an equilibrium affinity readout. By probing tryptophan responses directly in solution, the approach enables quantitative determination of dissociation constants without labeling or immobilization ...
Jianghao Shen, Qiangqiang Wang, Fan Wu, Yang Gao, Lu Lan, Pu Wang   +5 more
wiley   +1 more source

Glutamine Deprivation Triggers Tribbles Homolog 3 Dependent G‐Quadruplex Resolution to Maintain DNA Repair and Tumor Survival

Advanced Science, EarlyView.
Glutamine deprivation triggers transient DNA damage yet activates adaptive repair in hepatocellular carcinoma cells. We identify TRIB3 as a stress‐induced nuclear scaffold that associates with DDX5 and G‐quadruplex DNA atBRCA1 andRAD51AP1 promoters. TRIB3 loss increases G4 accumulation, suppresses HR gene transcription, elevates γ‐H2A.X, and sensitizes
Qiang Ji, Xuedan Sun, Zhangran Sun, Mengfan Li, Xinyu Cheng, Shuai Tian, Rick F. Thorne, Jinming Li, Guangzhi Liu, Mian Wu, Xiaoying Liu   +10 more
wiley   +1 more source

The 10–23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions

Angewandte Chemie, EarlyView.
This review focuses on the 10–23 DNAzyme in diagnostics, spanning unregulated and regulated target‐recognition modes, and their functionalization across colorimetric, fluorescent, electrochemical, electrochemiluminescent, and intracellular biosensing strategies.
Connor Nurmi, Jake Brill, Sanne Roumans, Amal Mathai, John D. Brennan, Yingfu Li   +5 more
wiley   +2 more sources

Dendritic Nano‐Based Slippery Coating by Synergistic Mechanical and Electrostatic Interactions with Persistent and Exceptional Combats Thrombosis

Advanced Science, EarlyView.
A novel dendritic nano‐based slippery coating (DNSC), fabricated by encapsulating carboxyl silicone oil within amino dendritic silica nanoparticles and co‐embedding them in an epoxy resin matrix. The dendritic architecture enhances nanoparticle dispersion in the matrix and establishes mechanical interlock.
Shu Zhang, Yao Shen, Juan Liu, Qing Zeng, Yunze Ma, Shuping Chen, Taiyu Nan, Xiaoying Qiu, Jiayi Yu, Tao Fan, Guozhi Huang, Jihua Zou, Chengduan Yang   +12 more
wiley   +1 more source

Assessment of Thrombin Generation

European Oncology & Haematology, 2010
Thrombin plays a central role in haemostasis through both procoagulant and anticoagulant functions. Although measurement of thrombin generation in the laboratory has been possible for many years, recent advances in understanding a wider scope of physiological roles of thrombin as well as improvements in technology have resulted in methods that are less
openaire   +1 more source

Advanced Corneal Hydrogels: From Passive Replacement to Active Regeneration and Intelligent Interaction

Advanced Science, EarlyView.
This review charts the evolution of corneal hydrogels from passive replacements to active and intelligent systems, evaluating natural and synthetic materials while addressing design challenges such as transparency and strength. Advanced applications such as scarless wound healing, three‐dimensional bioprinting, and smart wearables are highlighted to ...
Xinwei Wang, Wen Zhou, Haotian Xue, Wenjun Xue, Guangping Gao, Bing Zhu, Hongyuan Song, Qingqiang Xu, Jing Zhao, Wei Shen   +9 more
wiley   +1 more source

A Photothermal‐Responsive PRP‐Loaded Hydrogel Engineered With Composite Nanobottle for Controllable Delivery of Growth Factors and Multifunctional Therapy of Diabetic Wounds

Advanced Science, EarlyView.
A photothermal‐responsive engineered platelet‐rich plasma hydrogel (CNB‐ePRP) incorporated with thrombin‐functionalized composite nanobottle was developed, which enables in situ PRP activation, on‐demand growth factor release, multifunctionality, and personalized antimicrobial loading for treatment of diabetic wounds. The CNB‐ePRP hydrogel demonstrated
Wen‐Qiang Qu, Hui‐Yun Gu, Si‐Min Zeng, Chang‐Jiang Liu, Xiao Yan, Ting Pan, Chao Jian, Xian‐Zheng Zhang, Ai‐Xi Yu   +8 more
wiley   +1 more source

Ultrasound Activated Piezoelectric Dural Patches to Drive Endogenous Neural Stem Cell–Mediated Repair Traumatic Brain Injury

Advanced Science, EarlyView.
This study presents a wireless, non‐invasive strategy for neural repair by developing a biodegradable piezoelectric dural patch that, under transcranial ultrasound, generates localized electrical fields to drive endogenous neural stem cells toward neuronal differentiation and functional integration.
Pengbo Zhou, Qingyuan Wu, Yang Wu, Runzhe Huang, Wei Li, Hanjie Niu, Hongtao Sun, Huiyu Liu   +7 more
wiley   +1 more source

Jointless Tongue‐Like Bioactuator for Multidirectional Motion Through Directional Electrical Stimulation

Advanced Intelligent Systems, EarlyView.
A tongue‐like bioactuator is developed using orthogonally aligned cultured skeletal muscle tissues. By applying directional electrical stimulation of varying strengths, the actuator achieves jointless, multidirectional movements. The device features a fully soft, skeleton‐free design, enabling biomimetic deformation and functional actuation, and offers
Xuankai Gao, Kouhei Okasaki, Hirono Ohashi, Takeshi Sakurai, Yuya Morimoto   +4 more
wiley   +1 more source

Thrombin Generation by Valproate

, 2019
Pathological plasmatic thrombin generation can lead to many complications such as thrombotic/atherosclerotic diseases. The contact phase of blood coagulation can be triggered by low plasma concentrations of lipophilic or negatively charged groups. There are two main proteases that can start the whole intrinsic coagulation cascade: activated factor 12 ...
openaire   +5 more sources